Module 7 Lab Report

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Broward College *

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BSC2010L

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Biology

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Dec 6, 2023

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docx

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5

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You must download this form to your hard drive in order to save your answers Module 8 Lab Report Photosynthesis Student Name: Part 1: In the experiment you ran at home, upload the pictures of your results. (You may have to add them in addition to the lab report if you can’t embed them directly on this document). For this lab report, plant A was placed in sunlight and plant B was placed in the dark cabinet. 1. When plant B was first placed in the dark cabinet or protected from light in some way, you had to check the plant after 20 minutes and remove any small bubbles you may have seen on the leaves. Why did you need to remove the bubbles after 20 minutes? To release the produced oxygen 2. Could you have used a dicot leaf for this same experiment? Why or why not? No, because it has two cotyledons. 3. Which sample, A or B had the greatest amount of bubbles on its surface? Leaf A. 4. Why was the presence of bubbles used as an indicator of photosynthesis? Oxygen was produced. 5. If you wanted to observe the consumption of CO 2 instead of production of O 2 , how would you alter the experiment? Watch the following video about a plant growth phenomenon called phototropism. Another is gravitropism. https://youtu.be/HmHvWDeTt7Y After watching the video, go for a walk or drive and look around your neighborhood or yard. See if you can find evidence of phototropism. Take a picture of it and upload with this report. You should look for
vines, plants growing in shady areas or plants growing on the trunks or branches of trees. Below are a few examples: 1. In your lecture course you learn about a plant hormone called auxin. What role does that hormone play in phototropism? It produces a curving of the stem to tip toward the light. This stem tips because the hormone moves the darker side causing the cells to grow larger than those in the light side. Light and Photosynthesis:
In the virtual lab you extracted pigments from algae in order to find what additional pigments the algae had that expanded its absorption spectrum. Using your broad understanding of photosynthetic pigments and the wavelngths of light they absorb, you will visit the following sight and compare the effects of different factors on the rates of photosynthesis. This virtual simulator will use formation of bubbles to indicate photosynthesis, just as you did in your own plant experiment at home. Simulator site: Photosynthesis Simulator Part 1 1. On the left side of the simulator, select the 40W power source. 2. Select 50cm distance 3. Clear light source. 4. When you are ready, click start. There is a clock at the top of the simulator screen, allow the clock to rotate twice. 5. Click stop after two rotations around the clock. Your data will show at the top. 6. Below the simulator is a table. Record your data in the table. (You will need to add a new row for each data set. Add a new row by clicking the plus sign in the bottom left corner of the frame). 7. After collecting the data for clear light, click on reset in the simulator. 8. Repeat the simulation for Red, Blue and Green Light, each one for two minutes like the clear. You can select the different light colors with the drop-down menu. 9. Record the data for each color of light. 10. Print and save your light data. Part 2: 1. Repeat the same simulation, except this time change the power source to 100W. 2. Collect and record all your data, save and/or print. After you have collected all your data, enter the data into Excel (or numbers if you have a Mac) and create two graphs. One for rate of photosynthesis at 40Watts and one for rate of photosynthesis at 100W. Both graphs and your table of data must be uploaded with your lab report. Be sure to use the right type of graph for your data.
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Answer the following questions using your data. 1. Which color of light produced the greatest rate of photosynthesis regardless of power source intensity? Blue 2. Which color of light produced the lowest rate of photosynthesis regardless of power source intensity? Clear 3. Did changing the light intensity from 40W to 100W increase or decrease the rate of photosynthesis? Increased
a. Explain why you think the rate of photosynthesis increased or decreased. It increased because there was more light available to drive the reaction. 4. The simulator also allows you to change the distance of the light source in relation to the plant. You can move it closer or further away. a. How does this relate to the changes in the diversity of plant life in different parts of the world? The equatorial regions have more direct sunlight so the plants there can carry out photosynthesis at a higher rate. As you move away from the equator sunlight becomes less available so not as many types of plants can grow unless they are adapted. Equatorial regions experience a higher amount of plant diversity while northern and southern regions experience less diverse plant life. b. Why are equatorial regions so lush with vegetation while more northern and southern regions are not? Equatorial regions have ideal conditions for vegetation growth such as large amounts of precipitation, warm temperatures, and direct sunlight throughout the year while southern northern and southern regions can experience little to no precipitation, experience cold climates during the winter, and have less sunlight during their seasonal changes. 5. Compare your graphs to the absorption spectrum of known photosynthetic pigments below. 1. The green light in your experiment most likely excited which pigment in the plant? Chlorophyll b 2. The red light most likely was responsible for exciting which pigment? Chlorophyll a 3. The blue light most likely was exciting which pigment? Chlorophyll a 4. What pigment in the spectrum to the left would be beneficial to the plant in the simulator in order to increase its absorption spectrum? Chlorophyll b