BIO 1A03 Post-lab 1 Assignment

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McMaster University *

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1A03

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Biology

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Feb 20, 2024

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BIOLOGY 1A03 Post Lab Assignment 1 1. Include a screenshot of your results from the virtual micropipette activity (your actual results and the ideal results must be visible in your screenshot). All four volumes should be accurately pipetted in the screenshot. (2 marks) 2. Which micropipette would you use to measure and transfer 14 ul? (1 mark P20 Micropipette

3. Fill in the table with the volume that each of the following micropipettes would dispense (3 marks) Micropipette P20 P200 P1000 Micropipette set to: 0 6 4 1 3 7 0 5 8 Dispensed Volume (ul) 6.4 ul 137ul 580ul 4. Fill the table below by indicating how you would set the micropipette to dispense the required volume of liquid. (3 marks) Micropipette: P20 P200 P1000 Dispensed Volume (ul) 13ul 79ul 1000ul Micropipette set to: 1 3 0 0 7 9 1 0 0

5. Using the figure below, identify a data point that indicates there are other variables affecting the relationship (e.g evidence that this is not a cause and effect relationship). Identify the data point clearly with a circle or label. (1 mark)

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6. Using a set of 10 stat points (you make up the values) create a scatterplot in excel that would be representative of a weak positive correlation . Include the correlation value in your data set. (2 marks)

7. Using a set of 10 data points (you make up the values), create a scatter plot in Excel that would be representative of a weak negative correlation . Include the correlation value for your data set (2 marks) 8. Attach the appropriately formatted scatterplot you created in tutorial 1A using the instructions in Excel Tutorial #1 and the data in table 1A-3 for diploid AMY gene copy number vs AMY1 Protein Concentration. You can copy graphs from excel and past as images directly into words. (4 marks)

9. What is the correlation coefficient r that you calculated for your scatter plot of diploid AMY1 Gene Copy Number vs AMY1 Protein Concentration in question 8? What strength of correlation is represented by the calculated correlation coefficient? (2 marks) r = 0.50999327, This number represents a positive moderate correlation. 10. What were your R^2 values for trial 1 and trial 2 of the methylene blue standard curve? Given that you are using the same solutions and equipment for both the trials, what could explain the different R^2 values between the two trials? (2 marks) My R 2 value for Trial 1 was 0.9972 and My R 2 Value for Trial 2 was 0.9995. There are many reasons as to why the R 2 values for the 2 trials were different such as: - Pipettes might have been slightly tilted while taking in water and methylene blue samples - The Pipettes might not have been properly calibrated. - Human Error 11. Please include the correctly formatted standard curve for your methylene blue standard curve experiment in Lab 1. Follow the instructions in the Excel Tutorial #2. Include the figure from the trial that has the highest R^2 value (4 marks)

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