Cordero_yeast write up

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School

University Of Arizona *

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181R

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Biology

Date

Feb 20, 2024

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pdf

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Uploaded by CoachGerbilMaster973

Melia Cordero 12/6/23 Question: What is the mating behavior between strains and what are the effects of the FUS1 gene in yeast reproduction? Results: Table 1: MAA Table 2: MCBADDIES Table one : The data is taken from the group MAA and they investigated the shmooing behavior of A, alpha, alpha+mutant 1, and A+alpha cells. We can observe that Alpha and A cells have no sign of shmooing when placed on a plate by themselves. There was a little bit of shmooing that occurred in the alpha+mutant 1 and the most shmooing in the alpha + A plate. Table two : shows the results from my group's plate growth mating assay. We investigated A, alpha, a+alpha, alpha+mutant 1, and mutant 1 in 3 growth plates with different conditions. We concluded that alpha cells need lysine, a cells need methionine, A+alpha cells are able to mate and grow by sharing DNA, and mutant 1 can survive in methionine. Interpret: From these results, we can conclude that shmooing occurs most when in the presence of the A cells. Since we know that shmooing is a sexual reproduction between two strands we can infer that when shmooing occurs there is an exchange of DNA that allows each cell to live within a growth medium that they were incapable of living in prior to reproduction. This was proven when the A+alpha cells were able to grow in the plate that was deficient in methionine and lysine. We can also infer that there is a relationship between the FUS1 gene in the A cells and sexual reproduction. Final Model: Model Explanation: Our model aims to explain the processes of yeast sexual reproduction and yeast shmooing. We drew out the two types of mating yeasts a and alpha as well as a + alpha in the mediums that they successfully grow in. We drew a more detailed picture of the reaction happening in the A+alpha cell plate. We inferred that the A cell that contained the FUS1 gene must have some type of cell receptor that would receive a signal from the alpha cells and trigger a shmooing cell response. This prediction is slightly supported by the data shown in the results of table one (group maa). Mutant 1 is a strain similar to the a cells meaning it contains the FUS1 gene, which is why we observe shmooing in both the alpha + mutant 1 plate and the A+alpha plate.

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