MMG 065 - A01 - Lab 1 - Zeiter

docx

School

University of Vermont *

*We aren’t endorsed by this school

Course

MICROBIOLO

Subject

Biology

Date

Feb 20, 2024

Type

docx

Pages

Uploaded by ElderSnow13344

MMG 065 A02 MMG 065 - Lab 1 Study Questions 1. Discuss how your predictions of the environmental cultures aligned with your observations: Originally, I had predicted that the keyboard in the Howe Library would be the most contaminated due to the fact that it received a lot of traffic during the day and changes hands often without being decontaminated. In lab, we discovered that the most contaminated surface was the student desk in Given E-131, which disproved my original hypothesis. This desk had approximately 4 different types of colonies and 32 colonies in the sample. The keyboard only had 2 types of colonies and 3 colonies in the sample. The desk also had several colonies that had very different characteristics, which contributes to why the lab group thought it was most contaminated. 2. Refer to Table 2. Based on your observations of the environmental culture plates, which plate was actually the most contaminated? Based on my observations, the sample of the student desk in Given E-131 was the most contaminated. This sample had 4 different types of colonies (punctiform, small (2x), and large), all with different and distinct characteristics. In the entire plate, there were about 32 colonies. 3. Why do you think that this was so? What conditions may have contributed to the relative contamination these surfaces? I think that the Given E-131 student desk was the most contaminated due to the several different types and quantity of colonies on the sample plate. Some were punctiform,

small, or large. Among the varying sizes, there were also colonies that were yellow, white, brown, fuzzy, raised, irregular, and circular. Due to this sample’s diversity compared to the rest of the samples, there is evidence that it was the most contaminated. The student desk may be the most contaminated because it is used by several people in a day, rarely decontaminated, and may encounter food, drink, laptops, phones, and other heavily contaminated objects. Meanwhile, the keyboard that I had originally predicted, probably only has microbes from hands. 4. How might you re-formulate your hypothesis based on today’s observations? Based on today’s observations, I would focus more on the criteria I am considering when creating my hypothesis. For example, I might focus more on the number of colonies, types of colonies, and characteristics that would make something “contaminated”. Before this lab, I was only considering what I considered to be “most dirty”, instead of considering in what conditions microbes grow, what they might look like, and how many types of microbes a surface may come in contact with in any given day. 5. As you consider the ubiquity of microorganisms and your future career, what do you see as the most significant implications? The knowledge that microbes are everywhere is going to be a serious consideration in my future profession. As seen in lab, microbes can grow in almost any location, and under many different conditions. Healthcare-acquired Infections (HIA) can increase morbidity and mortality and are very significant implications of not using proper technique. Therefore, it is very important to always use proper decontamination and aseptic technique when working with samples or patients. If that is overlooked, microbes could find their way into patient’s wounds, catheters, IV’s or other important devices that could

seriously harm the patient’s ability to heal. Contamination could also cause improper patient diagnoses. It is important to not contaminate any tests or labs, as that could hinder the results or make the labs unusable. 6. What is aseptic technique? Why is it important in the lab or clinical environment? Aseptic technique is a set of safe practices that prevent the contamination of a patient, sample, or others by microbes. Aseptic technique is practiced by hand washing, disinfecting the workspace before and after each sample, using sterile materials, and demonstrating proper use of PPE. 7. In what ways do healthcare workers use the scientific method? Healthcare workers use the scientific method to predict, observe, explain, and test theories in order to provide correct diagnoses, and provide evidence-based care. Sometimes, healthcare workers need to hypothesize about possible causes of an infection or disease and then use the scientific method to determine the best course of treatment for that patient. 8. Observe the agar plates seen in this image. Which are labeled correctly? Select all that apply. There are no agar images to observe. 9. How do sterility and aseptic technique differ? Aseptic technique differs from sterility in that Aseptic technique is the set of practices that maintains sterility of the workspace and lab procedures. If we did not use aseptic technique, the lab procedure we carried out could not be considered sterile, as there would be contamination of other microbes in the experiment. 10. Identify instances when aseptic technique should be used.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Aseptic technique should be used any time we are collecting or processing a specimen, performing a procedure, working with patients, or observing/working with samples in the laboratory.

Related Documents

Copy of 3. Pre-lab quiz questions.pdf

chapter 7-8 answers to mock test.docx

3.jpg

Lecture HW 3 Skeleton Form and Function.docx

Lab HW 2 Histology of Supporting Connective Tissues.docx

Lab 1-CommunityStructure-Spring.docx

Determining Hereditary Nonpolyposis Colorectal Cancer(HNPCC)-Genetics Lab Report.docx

KEY Exam 1 for BYS 215 FA23 Lecture Exam 1.docx

Class 28- Translation Worksheet.docx

Bryson_Biostat_HW4.docx

Bryson_BIOL3000_Quiz1_2.docx

PS - BIO 111 Lab 5.docx

Recommended textbooks for you

3-2-1 Code It

Biology

ISBN:9781337660549

Author:GREEN

Publisher:Cengage

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

Essentials Health Info Management Principles/Prac...

Health & Nutrition

ISBN:9780357191651

Author:Bowie

Publisher:Cengage

Comprehensive Medical Assisting: Administrative a...

Nursing

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Cengage Learning

Surgical Tech For Surgical Tech Pos Care

Health & Nutrition

ISBN:9781337648868

Author:Association

Publisher:Cengage

Fundamentals of Sectional Anatomy: An Imaging App...

Biology

ISBN:9781133960867

Author:Denise L. Lazo

Publisher:Cengage Learning

SEE MORE TEXTBOOKS

Recommended textbooks for you

3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Fundamentals of Sectional Anatomy: An Imaging App...
Biology
ISBN:9781133960867
Author:Denise L. Lazo
Publisher:Cengage Learning