Biology CH 4 LAB

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School

Pennsylvania State University, World Campus *

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Course

101

Subject

Biology

Date

Feb 20, 2024

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docx

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10

Uploaded by MajorMetal2194

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Name: Stephanie Lasprilla Date: 02/17/2021 Virtual Microscope by BIONETWORK ONLINE EDITION http://www.ncbionetwork.org/iet/microscope / Instructions: You can use the “learn” tab to familiarize yourself with the virtual microscope. As you click on each of the microscope parts, read the pop-up to answer the following. 1. Identify each of the following by letter. On/Off Switch – K Eyepiece / Ocular Lens - C Arm - J Nosepiece - F Objective Lenses - G Stage - D Diaphragm - H Stage Adjustment - E Base - M Coarse Adjustment - L Lens Paper - A Fine Adjustment - N Immersion Oil - B Slide box - P Kimwipes - D 2. Explain what each of the following does:
- Kimwipes: o Brand of specialty paper-based cleaning wipes suited for cleaning laboratory equipment and instruments, camera lenses, fiber optic connectors and other sensitive electronic components. These are not lens papers and should not be used to clean microscopes as they can damage the lens. - Immersion oil: o Special oil used exclusively with a 100X or higher objective lens, typically at 1000X total power. Acting as a bridge between the glass lens and the glass slide, the oil concentrates the path of light to increase the resolution of the image - Diaphragm o Five hole disc housed under the stage that adjusts the amount of light passing through the stage opening. - Stage Adjustment Knob o Mechanical stage allows for adjustment of slide position forward, backward, and left or right. Go to the “EXPLORE” tab to view slides. You will need to use the coarse and fine focus and adjust the light to get clear views of the following. The slide can also be moved within the viewing field. Sample Slides - Letter E
3. Include a screenshot of the letter “e” at 10x. To take a screenshot, use the print screen on your keyboard or use your phone to capture the screen. Insert the image in the space below. Your image should clearly show that you viewed the “e” and focused it.
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4. What did you need to do before viewing the slide at 100x? When you switch to a 100X lens, you must turn the nosepiece halfway, add immersion oi proceed to snap the lens into place. 5. A common mistake for beginners is thinking that the “e” just disappears at the high magnification. How would you explain to a younger student what happened to the e as you increase the magnification? As the “e” increased in magnification, you can clearly see the lines of the “e” In further d thought possible.
Plant Cells Return to the slide box and choose plant slides plant cells. Focus with 4x, 10x, and 40x. You may need to adjust the light and center your slide. 6. Include a screenshot of the plant cells at 40x.
7. Plant cells can be identified by their boxy appearance, where lines represent cell walls. Within the cell are green circles which are chloroplasts and a dark gray area which represents the nucleus. At 40x, how many individual cells can be seen in the viewing field? 15 Human Blood Return to the slide box and choose human blood Focus with 4x, 10x, and 40x. You may need to adjust the light and center your slide.
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8. Include a screenshot of the blood cells at 40x.
9. Blood cells can be identified by their round appearance. Unlike plant cells, they do not have cell walls. At 40x, how many individual blood cells can be seen in the viewing field? About 50 10. At 100x, you will see a cell that is larger and has a dark shape in it. This cell doesn’t look like the others because it is a white blood cell and it functions to protect your body against microbes. Red blood cells do not have a nucleus. White blood cell count is used as a diagnostic tool to determine if someone is sick. If you were fighting an infection, would you expect to have more or less WBC’s? Why? If I was fighting an infection, I would expect to find more WBC’s because White blood cell cells responsible for fighting off an infection. Therefore, the more WBC’s found, it is more that there is an infection present. Synthesis and Discussion
11. Compare blood cells to plant cells, include details about color, general shape, and size. Write this as a complete sentence or paragraph. Plant cells have a boxy like appearance and are a striking green in color due to presence chloroplasts. The box like structures vary in size and resemble bricks. Blood cells are sma circular in size and shape. Blood cells are scattered-like everywhere, different from the or plant cells. 12. Explore bacteria slides. You don’t need to screenshot them. Compare the bacteria slides to the blood cells and include details about their color, general shape, and size. Write this as a complete sentence or paragraph. Bacteria slides are abstracted/deformed in shape compared to blood cells. The bacteria c In color and are smaller than red blood cells.
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