Lab 5 OL pipetting

docx

School

Chamberlain University College of Nursing *

*We aren’t endorsed by this school

Course

252

Subject

Biology

Date

Feb 20, 2024

Type

docx

Pages

Uploaded by AmbassadorStraw13374

BIOS242, Week 5-OL Name: Lab 5: Pipetting Learning Objectives  Select the correct micropipette for its purpose.  Use the two stops of the pipette.  Explain pipetting techniques.  Perform a serial dilution.  Quantify the protein content in a sample with a Bradford assay. Introduction: Master pipetting virtual simulation lab In the Pipetting simulation, you will determine the amount of essential amino acids in genetically modified corn. Using a micropipette, you will prepare a serial dilution, and measure the protein concentration with a Bradford assay. Get to know the micropipette You will be introduced to the basics of pipetting as you dive right into the experiments. Step by step, you will learn how to adjust the volume, use the two ‘stops’ of the pipette, and find out which pipette to use for which volume range. You will also learn tips and tricks to make your pipetting more accurate. Perform a serial dilution Once you master the art of pipetting, you will dilute your protein solution 10.000 fold! While you progress through the lab, the technique will become more routine. After this mission, you will understand the benefits of serial dilutions, and be able to perform one by yourself. Measure the protein content in the sample In this mission, you will dye your proteins blue, and use your new skills to prepare a microplate for a Bradford assay. In this colorimetric assay, you shine light of a specific wavelength through the plate, and measure the absorbance in each well. Finally, you will compare the absorbances of your sample to a sample of known protein concentration. Will you be able to calculate the amount of essential amino acids in the corn cob you collected? 1

BIOS242, Week 5-OL Name: Questions: 1. Purpose: Why is proper pipetting important in a microbiology lab? Proper pipetting is important in a microbiology lab as it is important for precision. Proper pipetting includes changning out the tips to avoid contamination of a solution. It also includes serial dilution which is needed when we need to receive a very highly diluted solution from a very concentrated solution. 2. What are the three different types of pipettes that are commonly used in lab? P1000: 200-1000 microliters P200: 20-200 microliters P20: 2-20 microliters 3. List 4 safety features that you will need to follow when using pipette? - Before drawing up liquid you have to attach a sterile tip and press the plunger down to the first stop - To release the liquid, press the plunger down to the second stop and discard into the trash after each pipette - Avoid putting the pipette by your mouth - When collecting a sample hold the pipette vertically and carefully release the plunger into the liquid 4. Reflection: Write 5 sentences on what you learned from this simulation. What did you like and what was something that you would prefer not be a part of this simulation? This stimulation not only taught me about pipetting but also what I believe to be one of the most important things I learned from this stimulation is what sterilization is, which is the removal of all microorganisms. Pipetting allows the accurate results and measurements of a liquid when done properly. Finally, I learned how to analyze the protein content in a sample through the Bradford assay technique. The only thing I would change about the stimulation was the repeativness of putting a clean tip on the pipette, pressing it to the first stop after each new 2

BIOS242, Week 5-OL Name: tip, and throwing the tip away after each experiment. I felt as if I understood the importance of discarding the tip and putting a new one on the pipette after each liquid drawn up and did not need to keep doing it after a couple of times. Grading Rubric: Activity Deliverable Points Lab Report and Questions Complete lab report and answer questions  Purpose (2 point)  Questions (8 points)  Reflection (5 points) 15 All Lab Deliverables  Complete ALL lab work and lab report 15 3

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Related Documents

Aquatic_Ecology_Lab Peer Review Sheet.docx

Big Fat Stack.docx

Osmosis revised in person.docx

Chapter 10.docx

1.11 Quiz The Lymphatic and Immune System.docx

202 Week 04 Lab Population Thinking.docx

hw01-sol.pdf

Biology CH 4 LAB.docx

Activity 2- Folate metabolism.docx

BIOL 1104 - Practice Tests.pdf

2024_ProblemSet_1(1).docx

unit 1 A&P review.pdf

Recommended textbooks for you

Comprehensive Medical Terminology

Nursing

ISBN:9781133478850

Author:Jones

Publisher:Cengage

Microbiology for Surgical Technologists (MindTap ...

Biology

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Cengage Learning

Biomedical Instrumentation Systems

Chemistry

ISBN:9781133478294

Author:Chatterjee

Publisher:Cengage

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:9781305143531

Author:CURREN

Publisher:Cengage

Intro To Health Care

Health & Nutrition

ISBN:9781337338295

Author:Mitchell

Publisher:Cengage

Complete Textbook Of Phlebotomy

Biology

ISBN:9781337464314

Author:Hoeltke

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

Comprehensive Medical Terminology
Nursing
ISBN:9781133478850
Author:Jones
Publisher:Cengage
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Biomedical Instrumentation Systems
Chemistry
ISBN:9781133478294
Author:Chatterjee
Publisher:Cengage
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:9781305143531
Author:CURREN
Publisher:Cengage
Intro To Health Care
Health & Nutrition
ISBN:9781337338295
Author:Mitchell
Publisher:Cengage
Complete Textbook Of Phlebotomy
Biology
ISBN:9781337464314
Author:Hoeltke
Publisher:Cengage