_Lab Report 2_6_24

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Feb 20, 2024

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Enzyme Activity Two 1 INTRODUCTION: The Perfectly Picked Produce Company has recently been encountering an issue with their produce browning fast. Within the experiment we are conducting, we are hoping to find how the effects of PH level can change the outcome of the enzyme activity. How do environmental factors such as PH change the rate at which the enzymes react? If the PH is higher or lower than 7, the rate of the enzymatic activity will rise because PH levels change the enzyme's reaction time and its activity. METHODS: Our methods are based on the instructions that were given to us. First we made orange juice by first putting it through the food processor. We then strained the juice to take out all the pulp. We then filled a vial with 4 mL of the juice along with an acid, neutral solution, or a base, this is also when we added the enzyme. We then finally put the mixed solution into the colorimeter to get our absorption reading. RESULTS AND FIGURE: This test resulted in 3 data points. An absorbance of 85 for the acidic solution, 110 for the neutral solution, and an absorbance of 105 for the basic solution. This means that the neutral solution would have the higher enzyme activity. We know that enzymes are usually most active around a pH of 7 (Lin 2013). Our data from the last 2 experiments seem to support that. The below figure shows raw data from the colorimeter. There is one data point per bar, these do not

Enzyme Activity Two 2 show an average. DISCUSSION: Our findings did not support our hypothesis, so it would be beneficial for the perfectly picked produce company to leave their produce in a neutral solution. Most enzymes work at a pH

Enzyme Activity Two 3 of 7 (Lin 2013). This explains why we got the results that we did. Catecholase specifically works best at a pH of 7 (Guzik 2014). This also explains why different produce brown at different rates. pH of the produce affects the rate catecholase causes it to brown. Literature Cited Lin, H., Chen, W., & Ding, H. (2013). AcalPred: a sequence-based tool for discriminating between acidic and alkaline enzymes. PloS one , 8 (10), e75726. https://doi.org/10.1371/journal.pone.0075726 Guzik, Urszula & Hupert-Kocurek, Katarzyna & Marchlewicz, Ariel & Wojcieszyńska, Danuta. (2014). Enhancement of biodegradation potential of catechol 1,2-dioxygenase through its immobilization in calcium alginate gel. Electronic Journal of Biotechnology. 17. https://doi.org/10.1016/j.ejbt.2014.02.001 .

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