A Mysterious Death

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University of California, Berkeley *

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Biology

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Feb 20, 2024

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pdf

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Uploaded by MinisterStraw10784

Bio1A: Spring 2024 A Mysterious Death You suspect that the girl may have somehow been poisoned and ingested one of the following chemicals: Go and research each of the chemicals above and answer each of the following questions below: 1) How does each chemical a0ect cellular respiration? 2) Which one of the four molecules would most likely (and directly) produce the autopsy results above? 3) How might a young person come into contact with such a chemical? GRADING RUBRIC: • On-time submission (before 2/11 at 11:59pm): 5 points • Propose how each molecule aNects cellular respiration: 4 points • Correctly identifying which molecule produced the autopsy results: 5 points • Identifying how a young person might come in contact with the proposed molecule: 1 point Normal Levels Autopsy Finding Metabolite 100 µ mol 102 µ mol Glucose 25 µ mol 23 µ mol Pyruvate 75 µ mol 6 µ mol NAD+ 50 µ mol 383 µ mol NADH In the morgue, you find the report from the hospital. Glancing over it, you notice a narrative of the girl’s last hours: At 10 am, mother returns from the store to find girl vomiting. Not feeling well, and sleepy. Mother put girl to bed. Tenminutes later, she noticed that the child’s breathing was irregular and slow. Shetried to wake her daughter, but was not able to do so. The child became comatose. At noon, the girl was admitted to the hospital, with no heartbeat or spontaneous breathing. Autopsy Report • The girl died within two hours of first vomiting. • Tissues sections from lung, heart, & kidneys show massive cell death. • Staining with cellular dyes indicates that the mitochondria within the affected tissues were damaged. • More detailed analysis of heart cells showed that ATP levels were reduced in the mitochondria. • ATP levels in the cytoplasm of these cells, however, were normal. • In addition, Acetyl-CoA molecule levels were normal. • Levels of various sub-cellular components in the heart cells. Cyanide Sodium Azide Rotenone Arsenic

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