Personal lab report 3

 Laboratory notebook  Disposable gloves Procedure I. Before using the spectrophotometer, make sure it has been warmed up for at least 10 minutes. II. Blanking: 1x TE buffer is used to blank the spectrophotometer. III. Measure Absorbance: When analyzing DNA samples: IV. Spoon a little amount (1-2 L) into a cuvette or microplate using a pipette. V. Remove moisture by wiping. VI. The spectrophotometer needs it. VII. Make a note of the absorbance readings at 260 and 280 nm (and, if necessary, 320 nm to account for background light). Data Sampl e ID User Nam e Date and Time Nucleic Acid Unit A260 (Abs) A280 (Abs) 260/28 0 260/23 0 CS Sam 9/21/2023 2:17 PM DNA ng/u 6.643 14.43 6 1.5 1 Sample 1 Lab 9/21/2023 2:18 PM DNA ng/ ul 5.868 3.622 1.62 1 Sample 2 Lab 9/21/2023 2:20 PM DNA ng/ ul 6.736 4.179 1.61 1 Suspec t 3 Lab 9/21/2023 2:22 PM DNA ng/ ul 7692 5.333 1.44 1 Suspec t 4 Lab 9/21/2023 2:22 PM DNA ng/ ul 6.162 6.081 1.53 1 Suspec t 5 Lab 9/21/2023 2:25 PM DNA ng/ ul 6.081 3.987 1.53 1

at specific wavelengths (e.g., 260 nm and 280 nm) to determine DNA concentration and purity. Question 3 UV-Vis Spectroscopy Method UV-Vis spectroscopy is used to assess DNA quantity and quality. It works by assuming DNA molecules receive UV light at 260 nm. The sample's DNA content affects absorption. UV-Vis spectroscopy may also measure DNA purity by measuring the A260/A280 ratio, which reveals DNA contamination to protein contamination. A higher A260/A280 score indicates pure DNA, Unlike Proteinase K DNA Extraction