Chapter 6 Hands-On Lab Integument Histology

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Feb 20, 2024

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Hanna Heim Todd Ward Bio 2101 October 19, 2023 Chapter 6 Hands-On Lab Integument Histology Instructions In this exercise, you will be gaining “hands-on” experience with histology slides through a web-based resource called HistologyGuide .org . You will be navigating through a wide variety of tissue sections at various magnifications just as you would if you were using a physical microscope. For each tissue listed, you will be asked to locate and label a specific set of structures. To document your work, please follow these steps:  Locate the specific tissue/structures at the specified magnification.  Take a screenshot of the image by clicking on the down arrow next to “Select View” in the top-right of the microscope view.  Label the required structures on the image.  Click the blue box to insert your saved image file (or insert image and then label).  Save the completed document as a .pdf and upload it into the appropriate MyCourses Assignment Folder. Important Note on Academic Honesty All labelled images must represent your own original work and may not be copied from another student or images found online. Each image should include your initials in the corner using the same digital pen/tool used for labelling structures. Page 1 of 10

Skin Structure A. Thick Skin On the Histology Guide website, navigate to Slide Box > Chapter 11 Skin > Thick Skin > MHS235 @ the lowest magnification (approximately 1.1x). Take a screenshot of the tissue with all of the following structures in view. Label each structure listed, and insert your labelled image in the box below. 1. apical surface (unable to locate) 2. epidermis 3. dermis 4. basement membrane 5. dermal papilla 6. epidermal ridge 7. hypodermis Page 2 of 10

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B. Thick Skin - Epidermis In the Slide Box, navigate to Chapter 11 Skin > Thick Skin > MHS235 @ 10x magnification. Locate the epidermis. NOTE: Carefully explore your slides below and focus on a region on the slide you want to document. It is important that you locate a good section before you begin the labelling process. Take a screenshot of the tissue at 10x with all of the following structures in view. Label each structure listed, and insert your labelled image in the box below. 1. stratum corneum 2. stratum lucidum 3. stratum granulosum 4. stratum spinosum 5. stratum basale 6. dermis Page 4 of 10

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C. Thick Skin - Dermis In the Slide Box, navigate to Chapter 11 Skin > Thick Skin > MHS235 @ 10x magnification. Locate the dermis. Take a screenshot of the tissue at 10x with all of the following structures in view. Label each structure listed, and insert your labelled image in the box below. 1. stratified squamous epithelial tissue 2. basement membrane 3. papillary layer 4. reticular layer 5. areolar CT 6. reticular CT 7. dense irregular CT Page 6 of 10

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D. Thin Skin - Pigmented In the Slide Box, navigate to Chapter 11 Skin > Pigmented Skin > MHS236 @ 10x magnification. Take a screenshot of the tissue at 10x with all of the following structures in view. Label each structure listed, and insert your labelled image in the box below. 1. keratinocytes 2. melanocytes 3. stratum corneum 4. stratum granulosum 5. stratum spinosum 6. stratum basale 7. papillary layer of dermis 8. reticular layer of dermis Page 8 of 10

Accessory Organs A. Hair and Hair Follicle On the Histology Guide website, navigate to Slide Box > Chapter 11 Skin > Thin Skin > MH 090 @ 4x magnification. Locate a hair follicle. Take a screenshot of the tissue at 4x with all of the following structures in view. Label each structure listed, and insert your labelled image in the box below. 1. hair follicle 2. hair shaft 3. hair bulb B. Merocrine Sweat Gland and Sebaceous Gland Navigate to Slide Box > Chapter 11 Skin > Hair Follicles > MH 0087b @ 4x magnification. Locate a view that contains both a merocrine and a sebaceous gland. Page 9 of 10

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Take a screenshot of the tissue at 4x with all of the following structures in view. Label each structure listed, and insert your labelled image in the box below. 1. merocrine sweat gland 2. lumen of merocrine sweat gland 3. sebaceous gland 4. hair follicle Page 10 of 10

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