PLANTSSS ASSIGNMENT

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Yorkville University *

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3180H

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Biology

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Feb 20, 2024

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Biol 3180H: Plants in Action Photosynthesis: Dependence on Light Characteristics Lab 5 Assignment This assignment consists of 8 questions, 13 marks, and is worth 6% of your final grade. Please provide no more than 2-3 sentences for each short answer question below. Light reaction 1. What is the optimal light quantity (distance from light) indicated in your photosynthesis simulation? Optimal Light quality (nm)? Optimal Light temperature (C o )? (3 marks) - Optimal light quality: 475 nm - Optimal light quantity: 30 cm - Optimal light temperature: 25 degrees Celsius 2. Propose a plausible explanation for the differences in the ATP response curves. Why are some curves a straight line while others have peaks and valleys? Hint: what is the difference between cyclic and non- cyclic electron flow? (1 mark) - The peaks in some curves (Run 1 and 2) are due to ATP production. Then the Calvin cycle uses ATP which results in a valley as glucose is formed in that period. In other curves the ATP rises continuously because there is no glucose production.

3. Is there an optimal temperature for photosynthesis? Explain why temperature decreases the rate of photosynthesis at either temperature extreme. (2 marks) - Yes, there is an optimal temperature for photosynthesis as it is an enzyme specific process (E.g. Rubisco). At extreme high temperature the enzymes denatures whereas at extremely low temperature the enzymes get deactivated decreasing or halting photosynthesis. Dark reaction 4. What is the optimal stroma pH for photosynthesis? (1 mark) - 7 to 8 pH 5. Propose a plausible explanation why stroma pH affects the rate of photosynthesis. (1 mark) - Stromal pH regulates the Rubisco enzyme which is involved in the carbon fixation. Changes in pH impacts the structure of rubisco. 6. Why are ATP and NADPH both necessary for carbon fixation? (1 mark) - ATP and NADPH serve as a source of energy, reducing themselves (losing electrons) to convert phosphoglycerate 3 acetate molecules into Glyceraldehyde-3-phosphate. 7. What are the effects of different ATP and NADPH concentrations on the rate of oxygen production and glucose production? (2 marks) - High ATP and NADPH concentrations refers that there is abundant energy for light and dark reactions to function and therefore oxygen and glucose production is likely to be high. Low ATP and NADPH concentration mean that there isn’t enough energy for the light and dark reaction to occur, so it is likely to be linked with a decrease in oxygen and glucose production. 8. If oxygen production is used to measure the rate of photosynthesis, and both oxygen and glucose production vary in response to outside factors, what can we conclude about the rate of photosynthesis? Hint: is the rate constant across species? Time? Space? Explain with the help of a real or imaginary example. (2 marks) The rate of photosynthesis is not constant across species, time, or space. For example, a plant A growing in high light intensity may produce more oxygen than a plant B growing in low light intensity but consider plant A would be less effective at efficiently utilizing oxygen compared to plant B. In such a case plant B will have a higher glucose production despite growing in low light, which concludes that there as other factors such as time, space, and specie efficiency matters.

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