Lab 1 worksheet-brooke finch

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Feb 20, 2024

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Brooke Finch Professor Rawls Microbiology 1/16/2022 Lab 1 worksheet Microbiology Lab Safety Orientation Video Questions: 1. List 2 measures that you can take to help accomplish the goals of primary containment. Proper microbiological techniques include the safe transport and disposal of cultures along with the correct use of personal safety equipment such as gloves and safety goggles. 2. Define secondary containment . Deals with protecting the outside environment from exposure to infectious organisms. It depends principally on the design of the laboratory and the availability of equipment, laboratory workers should maintain the labs safety features, such as keeping a closed door closed or leaving an exhaust fan on. 3. How should one dress prior to microbiology lab? Specifically, what type of shoes should be worn and what types of clothing elements should be avoided? Always wear closed toe shoes because open toed shoes can lead to injuries and potential liquids spilling. Avoid clothing with baggy sleeves that could catch fire. Dress with closed toe shoes, hair up, and sleeves that are tight fit to your arms. 4. What 3 pieces of personal protective equipment (PPE) should be worn while working in the microbiology lab? Always wear a lab coat, gloves, and safety goggles. The lab coat should only be worn in the lab.

5. Where should you dispose of plastic, non-reusable items such as gloves, petri dishes, and plastic inoculating loops? You dispose of them in the biohazard container. Reusable supplies such as culture test tubes and glass pipettes should have all labels removed before being placed in a rack or container designated for autoclaving. 6. How should you clean up a spill involving live microorganisms? Specifically, how should the spill be covered, how many minutes does the disinfectant need to be in contact with the spill material, and where should spill materials be disposed of? In the event of a spill, notify your instructor and your lab partners immediately. With your instructor’s approval, cover the spill with paper towels and saturate the towels with disinfectant. After 15 to 20 minutes, carefully wipe the spill up and discard the paper towels into the biohazard container for autoclaving. General microbiology safety video: 7. ( Be sure to answer both parts! ) How many seconds should you wash your hands for? As a reference you should be able to sing "Happy Birthday" to yourself ____ times during this time frame. You should wash your hands for at least 20 seconds which is the time it takes to sing the “Happy Birthday” song twice! CDC First Hand washing Video Questions: 8. List four instances when you should wash your hands . 1. After you use the bathroom 2. Before you touch or eat your food 3. After you play with or touch your pet or any animal 4. After you cough, sneeze, or blow your nose

9. How much hand sanitizer should be applied to the hands (visual reference) and for how long should you rub the hand sanitizer on your hands? You should use enough hand sanitizer to wet both hands completely. About a dime to a quarter size of hand sanitizer. You should rub it until your hands or dry, so about 20 seconds. CDC Second Hand washing Video Questions: 10. How many times do people typically touch their eyes, nose, and mouth every hour without realizing it? About 25 times every hour.

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