This graph shows the effect of temperature on ALP activity. The temperature increases to 32 °C
and then starts to decrease after that temperature.
Table 3
The enzyme activity average and standard deviation increase as the temperature increases.
Notebook questions
1.The enzyme that was used was alkaline phosphate.This was used because ALP is a Catalyst for
hydrolysis.
2.The para nitrophenol created the color change which was caused by the hydrolysis
3.The alkaline buffer was used in part 2 to measure catalytic rate and the optimal ph and
temperature was used for part 1.
4.It was essential to wait to add the enzyme because it gives enough time to denature.
5.
Lynggaard, L. S., Rank, C. U., Hansen, S. N., Gottschalk Højfeldt, S., Henriksen, L. T., Jarvis,
K. B., Ranta, S., Niinimäki, R., Harila-Saari, A., Wolthers, B. O., Frandsen, T. L., Heyman,
M., Schmiegelow, K., & Albertsen, B. K. (2022). Asparaginase enzyme activity levels and
toxicity in childhood acute lymphoblastic leukemia: a NOPHO ALL2008 study. Blood
advances, 6(1), 138–147.
https://doi.org/10.1182/bloodadvances.2021005631
Nakanishi, H., Onose, S., Kitahara, E., Chumchuen, S., Takasaki, M., Konishi, H., & Kanekatsu,
R. (2011). Effect of environmental conditions on the α-glucosidase inhibitory activity of
mulberry leaves. Bioscience, biotechnology, and biochemistry, 75(12), 2293–2296.
https://doi.org/10.1271/bbb.110407
Lee, D. W., Song, J. A., Park, H. S., & Choi, C. Y. (2022). The effects of low pH and high water
temperature on oxidative stress and cell damage in juvenile olive flounder Paralichthys
olivaceus: comparison of single and combined environmental conditions. Fish physiology and
biochemistry, 48(5), 1251–1264.
https://doi.org/10.1007/s10695-022-01112-1
Vimalraj S. (2020). Alkaline phosphatase: Structure, expression and its function in bone
