Enzymes Lab Log 5

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Enzymes Lab Log 5 Florida International University October 9 th , 2023 Antonio Mendoza Daniel Hidalgo Angelo Martin Andy-Linares Aballe Group 6 1. The Lock & Key model is when the shape of the substrate and the confirmation of the active site are complementary to one another. The Induced-fit model is an enzyme that undergoes a confirmational change upon binding to substrate. The shape of an active site transforms to become complementary to the substrate. 2. Factors that affect enzyme function are temperature, pH, and enzyme concentration. 3. Denaturation of enzymes is when the enzymes shape changes, decrease/prevent substrate from binding to active site = no reactions, at times it is reversible.\ 4. Question: Will the amylase sources Bacillus licheniformis & Homo sapiens have different coloration between each other? 5. Approach: By taking each of the amylase samples and pour the starch into the amylase then each tube that contains each of the amylase will be put into different temperatures for 2 minutes then take out the amylase tube then pour it into the plate and pour iodine into, repeat the step for a total of 10 minutes for both amylase sources. 6.

Expected Results: 7. The dependent variable is the color of the amylase source, and the independent variable is the temperature. 8. The data will show a relationship between the two variables since one directly affects the other since as the temperature changes the colorization of the amylase will also change. 9. A line graph should be used since the data is measured in time; the data is dealing with the time it takes to break down the starch. 10. I predict to observe the dark coloring of the amylase enzyme. Amylase Source Temperature ( ) ℃ Time Taken to Break Down Starch (min) Bacillus licheniformis Group 6 0 10 Minutes 25 10 Minutes 55 8 minutes 85 4 Minutes Homo sapiens Group 3 0 10 Minutes 25 4 Minutes 55 6 Minutes 85 10 Minutes

11. 0 2 4 6 8 10 12 Bacilus lichformis vs. Homo sapien Amylases Bacillus lichformis Homo sapien Temperature (0,25,55,85) Minutes Analysis: 1. Results: Refer to No. 11 for graph. The graph is interesting it seems that at different temperatures the enzymes do a better job at breaking down the starch such as bacillus lichformis at 55 to 85 degrees Celsius had a more effective job at breaking down enzymes, while homo sapien was 25 to 55 degrees Celsius at breaking down starch. There is a relationship between the two variables since it varies for both amylases, yet temperature does indeed influence both. At different temperatures one treatment or amylase is higher than the other. 2. Discussion: The results did show how in different temperatures the two different amylases work differently. It seems that at higher temperatures the bacillus lichforms works more efficiently while the homo sapien works more efficiently at lower temperatures such as 25 and 55 degrees Celsius.

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3. Improvements: If the experiment was repeated I and my team would have done a better job at pouring the starch into the amylase then the amylase into the plate, the time constraint made it difficult to focus so that is one thing we could have improved on was timing and quickness. 4. Future Directions: How long will it take the amylases to break down protein and what color will appear?

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