Tutorial 4 Assignment Template

Fall 2023 Tutorial 4 – Inheritance & Information Transfer BIOL 1103 Tutorial 4 Assignment Full Name: Student #: Lab section: Although the tutorial work may be completed in groups, all work submitted must be completed individually. It is important that you do not copy from the lab manual or from each other. DO NOT plagiarize! Unless otherwise specified in the question, all answers should be written using complete sentences, paragraph style. Part I: Mendelian Inheritance Colour blindness is an X-linked recessive disorder characterized by the inability to either differentiate between colours or see colour at all. Cystic fibrosis is an autosomal recessive disorder characterized by the abnormal secretion of thick mucus rather than thin. The resulting thick mucus can cause adverse effects in the lungs, pancreas, liver, kidneys, and intestines. Often men afflicted by this condition are rendered infertile. Solon ( ♂) is colour blind and is a carrier for cystic fibrosis and his partner, Gwynevere ( ♀) , is a carrier for both colour blindness and cystic fibrosis. Solon and Gwynevere have 4 offspring: Priscilla ( ♀) , Reah ( ♀) , Logan ( ♂) , and Andre ( ♂) . Priscilla is a carrier for colour blindness and has cystic fibrosis. Reah has colour blindness and is a carrier for cystic fibrosis. Logan does not carry the mutation for colour blindness but is a carrier of cystic fibrosis. Finally, Andre is both colour blind and is afflicted by cystic fibrosis. Using the information provided above, answer the following questions regarding the family’s genetics. IMPORTANT: Use the following notation to answer all questions: Individuals with colour blindness are delineated by X b , whereas X B is used for individuals without colour blindness. Individuals with cystic fibrosis are delineated by f , while those without cystic fibrosis are delineated by F . 1. What are the genotypes of Solon and Gwynevere (2 marks) ? The genotype of Solon (♂)- FfX b Y The genotype of Gwynevere (♀)- Ff X b X B 2. What are the genotypes of Priscilla, Reah, Logan, and Andre (4 marks) ? The genotype of Priscilla (♀)- ff X b X B The genotype of Reah (♀)- fF X b X b The genotype of Logan (♂)- FfX B Y The genotype of Andre (♂)- ffX b Y 1

Fall 2023 Tutorial 4 – Inheritance & Information Transfer BIOL 1103 3. Construct a dihybrid cross showing the possible genotypes of Solon and Gwynevere’s offspring (4 marks) F X b f X b FY fY F X b FFXbXb fFXbXb FFXbY FfXby f X b FfXbXb ffXbXb FfXbY ffXbY F X B FFXbXB FfXbXB FFXBY fFXBY f X B FfXbXB ffXbXB FfXBY ffXBY A total of 16 possible offspring. 4. Based on the dihybrid cross you constructed in the previous question, state the probabilities ( as a percentage ) of Solon and Gwynevere’s having offspring with the phenotypes below (6 marks) . a. An offspring without any genetic mutations ( i.e., homozygous dominant for both normal vision and cystic fibrosis). 1/16=6.25% b. An offspring with cystic fibrosis. 4/16=25% c. Priscilla being a carrier for colour blindness and cystic fibrosis. 2/16=12.5% d. Reah having colour blindness and being a carrier for cystic fibrosis. 2/16=12.5% e. Logan having normal vision and being a carrier for cystic fibrosis. 2

Fall 2023 Tutorial 4 – Inheritance & Information Transfer BIOL 1103 If the thiamine is deleted from the chain, then the frameshift mutation will occur. This mutation alters the production of all amino acid sequences, so all the rest of the proteins are going to be incorrect. 8. A mutation occurs in the original DNA sequence for protein X strand such that the thymine nucleotide in position +48 is changed to a cytosine. What is the effect of this mutation ( 2 marks )? GAT in DNA will lead to the production of GAU in mRNA, which stands for aspartic acid production. If we replace U with C then nothing will change, aspartic acid will still be produced, so it is a silent type of mutation. 4 This assignment is due 1 week after completing your Tutorial 4 session, but must be submitted to Brightspace on the hour (i.e., 8 AM or 1 PM, depending on your section) prior to your next session. Deadlines therefore vary by section. Best of luck on exams! If you have any questions about the lab component, please email the lab coordinators. Otherwise, we hope that you enjoyed the BIOL 1103 labs 😊