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Question 29 Correct Mark 1.00 out of 1.00 " Flag question Some Steps Involved in Creating Recombinant DNA Plasmid DNA is cut at the restriction site. Foreign DNA is cut at the restriction site. Plasmid DNA is opened and sticky ends are formed. Foreign DNA restriction fragments are isolated Target sequence in the plasmid is recognized by the restriction enzyme. Target sequences in the foreign DNA are recognized by the restriction enzyme. 7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site. 8. DNA ligase splices the foreign restriction fragment and the plasmid together. Al e Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA. a. 6,2,4,5,1,5,8,7 bin6,2.4,7.8 5. 15 c. 51,36,2,4,8,7 d 5,1,3,6,2,4,8V Your answer is correct. The restriction endonuclease recognizes a specific sequence. The same endonuclease is used to cut the plasmid DNA and the foreign DNA from another organism. The plasmid is cut at one restriction site to open this small circle of DNA. The foreign DNA is cut at two restriction sites, on either side of the gene of interest. The small fragments of DNA created from this cleavage are called restriction fragments. The ends of open plasmid are sticky and can base-pair with the restriction fragments of the foreign DNA. An enzyme called DNA ligase can be used to splice or glue these fragments together. The correct answeris: 5,1,3,6,2,4,8