Fruit Fly Lab Report

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Jun 7, 2024

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Fruit Fly Lab Report Instructions: In this virtual lab activity, you will investigate the preferences of fruit flies and compare their tactic responses to different environmental stimuli. After which, you will perform chi-square hypothesis testing to compare your data on fruit fly behavior. Submit your lab report to your instructor when completed. Title: Fruit Fly Lab Report Objective(s): Decide if the sex of fruit flies affects their response to stimuli Create your objectives based on the goals of the lesson and lab activity. Introduction–Fly Behavior in a Choice Chamber In this laboratory activity, you will observe the behavior of fruit flies in a choice chamber. Review a similar experiment scenario below and answer the pre-lab reflection questions. A student constructed a choice chamber by cutting off the bottoms of two plastic bottles (of the same size) and using clear plastic tape to tape the bottoms of the bottles together. She labeled one side A and one side B. She tapped 20 fruit flies into the choice chamber by placing a cap on one end and a funnel on the other end of the chamber. She tapped the flies into the chamber from a culture vial. After transfer, she replaced the cap and funnel with cotton balls. She placed five drops of distilled water on each of the two cotton balls and laid the chamber on a white piece of paper. After five minutes of undisturbed time, she counted the number of flies at each end of the choice chamber. The data she collected is below: Side A Side B 5 minutes 11 flies 8 flies 10 minutes 9 flies 12 flies 1. Was there an overall orientation movement after 10 minutes? Explain the reason. a. There wasn’t a shift towards side B within 10 minutes. The difference wasn’t significant. This could be because both ends of the tube receive stimuli in measure leaving a comparable number of flies on each side. 2. Describe the importance of using distilled water at both ends of the chamber? a. It is important to use distilled water to avoid any contamination, from chemicals or compounds that could impact the accuracy of the experiment and compromise the validity of the results.

3. Explain why the choice chamber was placed on the white piece of paper. a. The tube was placed on paper for two reasons. Firstly, using a background makes it easier to spot and count the flies inside the tube. Second, if it had been a different color, like yellow, it could have potentially influenced the behavior of the flies 4. Identify two kinds of stimuli that could alter the results within the choice chamber. a. Light and heat are two factors that could influence the outcome of the choice chamber. If the student conducted the experiment in a room, with a controlled temperature and ensured that both sides of the chamber were equally illuminated by light these factors might have affected the results of the choice chamber Hypothesis: Create a hypothesis based on the investigative question you selected in the fruit fly virtual lab. Be sure to use an if/then statement format for your hypothesis. If both male and female fruit flies are subjected to stimuli, then their reactions to the stimuli will be different. Procedure: Access the virtual lab and complete the procedures provided for your selected investigative question. Provide a brief summary of the steps you followed during the experimental procedure. Materials: Fruit Fly Virtual Lab Fruit Fly Virtual Lab Summary of Steps: Place the heat lamp on one side of the tube. Let flies into the tube. Keep track of the number of female flies, on each side of the tube every 15 seconds. Repeat this procedure for each trial. Modify the variable in each test Variables: List your controlled variables, independent variable, and dependent variable for each trial of the investigation. Hint: For this experiment, the controlled and dependent variables are the same in each trial, but the type of independent variable is changes. Remember, controlled variables are factors that remain the same throughout the experiment. An independent (test) variable changes so that the experimenter can see the effect on other variables. The dependent (outcome) variable will change in response to the test variable. Controlled variables: Different types of flies are exposed to stimuli in the tube Dependent variable: Number of female and male flies of each side of the tube.

Independent variable: Sex of the flies. Trial 1: A 9f 7m B 2f 2m, A 11f 9m B 0f 0m Trial 2: A 12f 8m B 6f 4m, A18f 12m B 0f 0m Trial 3: A 9f 2m B 6f 13m, A 1f 1m B 14f 14m Trial 4: A 8f 2m B 7f 13m, A 2f 1m B 13f 14m Trail 5: A 10f 0m B 5f 15m, A 0f 0m, B 15f 15m Data: Create a data table to match the investigative question you selected for this virtual lab. Conclusion: Write a conclusion statement that addresses the following questions: ● Describe the responses of your fruit flies to your experimental stimuli. ● What taxis were exhibited by the fruit flies during your experiment? ● Does your data support or fail to support your hypothesis (include examples)? ● If this lab was completed in a laboratory, instead of virtually, what possible sources of error could have impacted the behavior of the fruit flies? During each experiment fruit flies typically showed a preference for one side of the tube over the other. Initially there might be males on one side or more females, on the other. By the 30 second mark nearly all if not all of them would have gathered on one side or the other. During the two trials the flies gathered around the sections linked to brightness, the side with the lamp and the side with the paper. In the three trials the flies consistently gravitated towards the portion of the tube containing vinegar. The data does not align with what I expected. Initially there was a separation, by gender as evidenced in the 15 seconds of trial 3 where 9 females and 2 males chose side A while 6 females and 13 males opted for side B. However this pattern did not stay. By the 30 second mark all the flies regardless of gender gathered on one side or the other. Lab Reflection Questions Answer the reflection questions, using what you have learned from the lesson and your experimental data. It will be helpful to review chi-square hypothesis testing in the statistical tests and data analysis section of your AP Science Practice Skills Tutorial . Answer questions in complete sentences. 1. Make a claim explaining why the fruit flies behaved as they did in your experiment. Justify your claim by providing evidence and reasoning to support your claim.

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