MLT 141
Clostridium difficile
Enzyme Immunoassay
Instructions:
Read the package insert and procedure card. Answer the questions below, interpret the +/- QC and patient Immunocards already performed for you and document the results below. Submit the completed document in
the appropriate assignment drop box. Interpret +/- Quality Control along with the Patient.
Circle:
Positive QC: ACCEPTABLE NOT ACCEPTABLE (.5pt)
Negative QC: ACCEPTABLE NOT ACCEPTABLE (.5pt)
Patient Name: ____Sparks, Cody
_____________ MR#: __93485
_______________
Interpretation: ________
Positve (+)
_____________ (1pt)
Kit Lot#: ________N/A__________________ Expiration Date: _____N/A______________ (1pt)
1)
What type of Immunoassay is this test(1pt)? qualitative enzyme immunoassay
2)
If the wash step in the procedure was omitted, what effect would this have on patient results(1pt)? any unbound antibody would remain in the test well, this would react with the substrate and possibly produce false positive results
3)
Draw an example of each result. Label each: Toxin A/B (antigen), capture antibody (Anti-Toxin A/B), Enzyme-labeled conjugate and substrate.
Positive (1pt):
Negative (1pt):
Failure to Wash Adequately (1pt):
4)
Describe the spectrum of disease (symptoms) caused by Clostridium difficile (1pt).
Partially formed or watery diarrhea, fever, foul stool odor, abdominal pain, recent antibiotic use
5) What is the cause of the diarrheal illness caused by C. difficile (1pt)
?
a.
Contaminated food c. Antimicrobial therapy
b.
Contaminated water
d. Shell fish
6) T/F C. difficile has emerged as a major nosocomial enteric pathogen in hospitals, nursing homes and extended care facilities (1pt). True
7) What is the predominant pathogenic mechanism that causes C. difficile dysentery (1pt)?
a. Exotoxin
b. Toxin A
c. Toxin B
d. Capsule
