Review Test Submission_ Final Exam Review Questions –practice results

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Calhoun Community College *

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2021

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May 24, 2024

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 1/24 Review Test Submission: Final Exam Review Questions (SP2021) MLT_141_H07 - MLT Microbiology I Course Content ... Cumulative Final Review Review Test Submission: Final Exam Review Questions User Josephine Alease Sullivan Course (SP2021) MLT_141_H06/H07 - MLT Microbiology I Test Final Exam Review Questions Started 5/2/21 10:11 PM Submitted 5/3/21 12:08 AM Status Completed Attempt Score 133 out of 145 points Time Elapsed 1 hour, 57 minutes Question 1 Quantify the colony growth on this SBA plate. Question 2 Home Student Resources 1 out of 1 points 1 out of 1 points Josephine Sullivan

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 2/24 Interpret the Gram Stain reaction of the organism in the image. Question 3 What are the clear areas inside this Gram positive bacilli? Question 4 What type of hemolysis pattern does the organism in the image produce? Question 5 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 3/24 What type of hemolysis pattern does the organism in the image produce? Question 6 What type of hemolysis pattern does the organism in the image produce? Question 7 >100K CFU/mL of a GNB were isolated on MAC agar from a urine specimen. Biochemical results are as follows: Glucose: Acid, gas produced Indole: nega±ve Urea: posi±ve PDA: posi±ve H2S: posi±ve The organism is MOST likely: 1 out of 1 points 0 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 4/24 Question 8 A GNR, isolated from a stool culture, produces a yellow colony on TCBS agar, a yellow slant/yellow bu² on TSI and a pink slant/yellow bu² on KIA is able to ferment: Question 9 A nasal swab from a 2 year old was plated on SBA, CA and MAC. The direct gram stain showed Moderate WBCs and 3+ gram negative coccobacilli. After 18 hours of incubation, no growth was present on the SBA or MAC plates. The CA had 3+ growth of small, grey colonies. What assumptions about this organism can you make from this growth pattern? Question 10 A pa±ent has been on treatment for gastric ulcers caused by H. pylori for 4 months. Which of the following tests should NOT be used to determine the effec±veness of this pa±ent’s treatment schedule? Question 11 A sputum culture from an alcoholic seen in the ER grows gray, mucoid, stringy colonies on SBA. The isolate grows pink colonies on MAC agar. The colonies yield the following test results: Mo±lity: - Indole: - Glucose: + Oxidase: - Citrate: + VP: + What is the MOST likely organism? Question 12 An aerobic GPR with spores belongs to the genus: Question 13 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 5/24 An aerobic GPR with swollen, club-shaped ends is a suspected: Question 14 An aerobic, partially acid fast, branching, GPR is most likely: Question 15 An ideal specimen for the isolation of a catalase negative GPR displaying H2S in TSI and test tube brush morphology in gelatin is: Question 16 Colonies suspicious for C. diphtheriae on Tinsdale agar: Question 17 Escherichia coli is a lways pathogenic. Question 18 Listeria monocytogenes can be distinguished from Streptococcus agalactiae (Group B Streptococcus ) because it is: 1. catalase positive 2. gram positive 3. sodium hippurate positive Question 19 Nontreponemal tests can be used to screen for syphilis in which stage of the disease? There may be more than 1 answer. Question 20 Spontaneous abortion or stillbirth during pregnancy may result from an infection with: 1 out of 1 points 0 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 0 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 6/24 Question 21 The best procedure(s) to differentiate Bacillus anthracis from other non-pathogenic Bacillus sp. include motility and hemolysis production because B. anthracis is: Question 22 The etiological agent of anthrax is: Question 23 The pathogenic enteric organism that characteris±cally produces rice water stools causes disease because of: Question 24 What reac±on can be used to differen±ate Klebsiella pneumoniae from K. oxytoca? Question 25 Which agar contains bile salts and dyes to slow the growth of most nonpathogenic gram nega±ve bacilli and allow Salmonella spp. and Shigella spp. to grow? Question 26 Which of the following biochemical tests or physical characteris±cs can defini±vely determine if a gram nega±ve rod is a member of the Enterobacteriaceae or a true nonfermenter? Question 27 Which of the following characteris±cs differen±ate H. pylori from Campylobacter sp.? Question 28 Which of the following medium promotes the formation of the metachromatic granules in Corynebacterium diphtheriae ? 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 7/24 Question 29 Which stage of Lyme disease can not be diagnosed by serologic methods. Question 30 A 28 year old female developed bloody diarrhea a³er a picnic lunch of fried chicken. The enteric pathogen isolated gram stained as small loosely spiraled GNRs. From what atmosphere did you take the culture medium to make this gram stain? Question 31 A GNDC that turns purplish-black with oxidase reagent, turns glucose and maltose substrates yellow is most likely: Question 32 A SBA plate inoculated with a 1µL loop of urine grows 70 colonies of S. aureus. How many colony forming units (CFU) per mL of urine should be reported? Question 33 A TSI slant inoculated with an organism gave the following reac±ons: Alkaline slant, acid butt, no H2S, no gas produced This organism is MOST likely: Question 34 A TSI slant was incubated in CO2 for 24 hours at 37C. The media is completely yellow. How would the technologist interpret this reaction? Question 35 A carbohydrate fermentation test with phenol red as a pH indicator should be incubated at/in ___________________ 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 8/24 1. –25°C. 2. 35°C. 3. ambient air. 4. CO2. Question 36 A catalase negative, long, filamentous, gram positive, non-spore forming rod is most likely: Erysipelothrix Listeria Lactobacillus Question 37 A direct smear of pus from a surgical incision reveals WBCs and gram-positive cocci in clusters. The colonies on SBA are beta-hemolytic, white and butyrous. There is no growth on MAC agar. The organism bubbles with 3% H2O2, displays a smooth suspension on the slide Coagulase, but is tube Coagulase positive. The organism is most likely: Question 38 A fastidious gram-negative cocci turns purplish-black with the oxidase reagent, bubbles with the catalase test and produces acid in glucose only is potentially: Question 39 A gram negative coccobacillary organism isolated from CSF on CHOC agar resembles either a Kingella sp. or N. meningitidis . The single best test to distinguish between these two organisms is: Question 40 A gram stain of an abscess showed Many WBCs 4+ GPC and 3+ GNCB. The aerobic culture had the following results: SBA and Chocolate agar: 4+ Staphylococcus aureus No growth on MAC Thioglycolate broth: cloudy What should the microbiologist do next? 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&r… 9/24 Question 41 A medium useful for the isolation of pathogenic Neisseria is: Question 42 A microbiologist inoculates 4 sets of OF media (nega±ve control, glucose, lactose and fructose). One tube from each set is le³ open to the atmosphere. The other tube from each set is overlaid with sterile mineral oil. All 4 sets are placed in the CO2 incubator. A³er 24 hours all four of the open tubes are yellow. The closed tubes remained green. Which of the following statements explains these results? Question 43 A microbiologist suspects a Beta hemolytic colony growing on Streptococcus Select Agar (SSA) to be Streptococcus pyogenes, the organism that causes strep throat. Why wouldn’t the technologist consider a Staphylococcus species? Question 44 A non-spore forming GPR is isolated from a wound on a farmer’s finger. The following test results were observed: Catalase- negative TSI- blackening along the stab line Bile esculin- negative Motility- negative Gelatin- no liquefication and “bottle brush” growth This organism can be identified as: Question 45 A pa±ent complains of stomach pain a³er ea±ng. The physician suspects pep±c ulcers. What specimen will the laboratory probably receive to confirm the diagnosis? 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 10/24 Question 46 A physician has sent an expectorated sputum specimen, collected from an elderly female suspected of having pneumonia, to the laboratory. The gram stain reveals many gram positive diplococci and many WBCs. The isolate grows as an alpha hemolytic, mucoid, gray colony. It does not bubble with catalase reagent. The most appropriate action to take is: Question 47 A physician sent a sputum specimen collected from an older patient suspected of having pneumonia to the laboratory. The gramstain reveals many gram negative diplococci and white blood cells. The isolate grows on both SBA and CHOC agar, turns purplish-black with the oxidase reagent and bubbles with 3% catalase reagent. Acid is not produced in glucose, lactose, maltose and sucrose. The most appropriate action to take is: Question 48 A positive DNase test would be indicated by: Question 49 A positive reaction for nitrate after the addition of zinc dust appears: Question 50 A rapid test for differentiating Streptococcus pneumoniae from other alpha-hemolytic streptococcal colonies is: Question 51 A small beta hemolytic gray colony was isolated as the predominant organism from a throat culture. The microbiologist should perform: Question 52 A small, gray, gamma-hemolytic colony which appears as gram positive cocci on the Gram stain is isolated from a decubitus wound specimen. The PYR test turns red after addition of the PYR reagent. It blackens a bile-esculin agar slant and grows in 6.5% NaCl. It is a: 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 11/24 Question 53 A sputum culture from a 10 year old cys±c fibrosis pa±ent is received in the laboratory. The sheep blood and chocolate agars grew heavy growth of a mucoid, Oxidase –posi±ve gram nega±ve rod with a blue-green pigment and a grape like odor. The MacConkey agar shows heavy growth of a mucoid NLF. The culture also included an OFPBL agar (oxida±ve-fermenta±ve base, polymyxin B, bacitracin, lactose), which grew light growth of a yellow colony. The organism growing on the OFPBL media will probably be: Question 54 A technologist is interpreting a urine culture and is documenting the colony morphology from the growth on sheep blood agar. Approximately 65 colonies are present of a large, flat, grey colony. The sheep blood around the colonies is completely destroyed. What is the proper documentation of the growth? Question 55 A technologist suspects Escherichia coli as the pathogen in a female patient’s urine culture. The specimen is contaminated with an over-abundance of normal genital ±ora and she doesn’t have an isolated colony on sheep blood agar on which to do the indole test. She decides to use a swab to pick up a colony from the MacConkey plate since all the normal ±ora was inhibited by the agar and isolated colonies of the lactose fermenting gram negative rod are easily accessible. Is this acceptable? Will the results be valid? Question 56 A urine culture SBA plate demonstrates >100K CFU/mL mixed gram positive genital ±ora (usual genital ±ora) and <15K CFU/mL of E. coli. What do these results indicate? Question 57 A urine culture was plated on a CNA and MAC bi-plate. Growth appeared on both types of agar. What do these results indicate? Question 58 A urine culture, on a 26 year old female, grew >100,000 CFUs/mL of a white, opaque, non- hemolytic colony. The colony stained as a gram positive cocci, bubbled with 3% H2O2, did 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 12/24 not clot plasma in the tube Coagulase test and grew up to the edge of Novobiocin disk. It should be reported as: Question 59 A urine specimen was collected in a physician’s o²ce on Friday afternoon. It was placed in the refrigerator for the courier to pick up and transport to the microbiology laboratory. The courier missed the specimen on his last run of the day. The specimen remained in the refrigerator until Monday morning. The courier picked up the specimen on his ³rst run of the day and transported it to the microbiology laboratory. On receipt, you will Question 60 A wound culture was set up on an elderly patient with a large bed sore. The aerobic plates showed no growth after 2 days of incubation. The thioglycholate broth was cloudy on the bottom portion of the broth medium only. What type of organism should the technologist suspect? Question 61 A yellow colony that appears as gram-positive cocci in clusters, bubbles with catalase reagent, and turns dark blue when rubbed onto a modified oxidase disk is: Question 62 After streaking a sheep blood agar plate with a throat swab, the purpose of stabbing the inoculum into the agar (or placing the plate in an anaerobic jar) is to: Question 63 An ONPG test for delayed lactose fermentation turns yellow. What does this indicate? Question 64 An Oxidase posi±ve, gram nega±ve rod demonstrates a ﬂat colony with aluminum/metallic sheen on the surface of the colonies. It is a nonlactose fermenter but demonstrates a green pigment that extends beyond the colonies on MacConkey. What addi±onal characteris±c is necessary to iden±fy the organism as Pseudomonas aeruginosa using rapid iden±fica±on criteria? Question 65 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 13/24 An acceptable specimen for the detec±on of the bacteria responsible for whooping cough is… Question 66 An isolate on chocolate agar from a pa±ent with epiglo´±s was sugges±ve of Haemophilus sp. Addi±onal tes±ng showed that the isolate required hemin and NAD for growth and was nonhemoly±c. The organism is MOST likely Haemophilus : Question 67 Besides respiratory tract specimens, what clinical specimen is useful for the sensi±ve detec±on of Legionella an±gen? Question 68 By which process do nonfermenta±ve rods u±lize carbohydrates? Question 69 Can an Indole test be performed on a colony taken from a MacConkey plate? Question 70 Enteric bacteria are common residents of the human __________. Question 71 Enterobacteriaceae are typically GNB or GNCB. Question 72 Enterobacteriaceae are typically glucose fermenters. Question 73 0 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 14/24 Enterobacteriaceae are typically mo±le. Question 74 Enterobacteriaceae are typically oxidase negative. Question 75 Enterobacteriaceae are typically unable to reduce nitrate. Question 76 Enterobacteriaceae typically do not contain LPS, an endotoxin. Question 77 Escherichia coli is Indole negative. Question 78 Escherichia coli is MR posi±ve, VP nega±ve Question 79 Escherichia coli is a non-fermenter. Question 80 Escherichia coli is citrate positive. Question 81 Escherichia coli is motile. Question 82 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 15/24 Question Examine the figure below of GPC characteristics. Can you determine why certain items are put in the same group? Identify each ladder by the characteristics it lists. ________________ #1 Beta #2 Alpha #3 Gamma (Non- hemolytic) ______________ #1 BE/PYR and Lancefield Group #2 CAMP and Hippurate #3 Optochin and Bile Solubility ______________ #1 Gram Stain #2 Catalase #3 Coagulase Question 83 Expectorated sputum is sent to the laboratory for culture from a patient with respiratory distress. The gram stain of the specimen shows many squamous epithelial cells (>25/lpf) and rare neutrophils (<25/lpf). The microscopic appearance of the organisms present include: Moderate GPC in chains and diplococci Moderate Gram negative diplococcic Moderate palisading GPR This gram stain is MOST indicative of: a pneumococcal pneumonia an anaerobic infection an Haemophilus pneumonia oropharyngeal ±ora (spit) 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 16/24 Question 84 Fever, leucopenia, disseminated intravascular coagula±on, and shock caused by infec±on with the Enterobacteriaceae are most strongly associated with which of the following? Question 85 Gram-positive lancet-shaped diplococci grow as an umbilicated, alpha- hemolytic colony. It is possibly: Question 86 How do microscopic and colony characteristics help categorize the gram-positive cocci? Question 87 If a microbiologist only had enough specimen to inoculate 1 media plate, which should he/she select? Question 88 If an organism shows healthy growth on Thayer-Martin, this suggests the organism is: Question 89 If the microbiologist is extremely busy and can’t immediately set up a stool culture, what temperature should the unpreserved specimen be held at until processing can be done? Question 90 In carriers of Salmonella typhi, the organisms are most likely to reside in which of the following sites? Question 91 Infec±ons caused by B-lactamase –posi±ve H. inﬂuenzae should be treated with which of the following? 0 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 17/24 Question 92 LIM media is used for selective isolation of: Question 93 Mannitol salt agar is used for: Question 94 Microorganisms associated with bacterial endocarditis include: 1. viridans streptococci 2. S. gallolyticus (Group D streptococcus) 3. Coagulase negative Staphylococcus sp. Question 95 No red color produced after the addition of the Nitrate Reagent indicates a negative test. Question 96 One day a³er a picnic featuring potato salad and chicken, 10 of the a²endees developed diarrhea. Two of them became sick enough to be hospitalized. A mo±le gram nega±ve rod that was nega±ve for lactose fermenta±on but posi±ve for H2S produc±on was isolated from stool samples of both pa±ents. Given this scenario, which of the following is most likely to be the cause of the diarrhea? Question 97 Partially lysed red blood cells under and around a colony on sheep blood agar appear as Question 98 Proper media for culture of a urethral discharge form a man include: Question 99 Select the gram nega±ve bacillus that can be described as indole nega±ve and H2S posi±ve. 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 18/24 Question 100 Select the organism(s) that are capable of growing on KVLB agar. There may be more than 1 answer. Question 101 Shigella spp. are characteris±cally… Question 102 Staphylococcus can be distinguished from Neisseria by performing a gram stain and a/an: Question 103 The 6.5% NaCl test is considered positive when one observes: Question 104 The causative agent of gonorrhea is an oxidase positive GNDC that: Question 105 The direct Gram stain of expectorated sputum reveals approximately 30 squamous epithelial cells and 10 WBCs per low power ³eld. You should: Question 106 The etiologic agent of actinomycosis is: Question 107 The expected colony count in suprapubic urine (kidney tap) from a healthy individual is: Question 108 The gram stain of a newborn’s CSF show gram positive pallisading rods. Small, translucent colonies with narrow zones of beta hemolysis appear on SBA after 24 hours in a CO2 0 out of 1 points 1 out of 1 points 0 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 19/24 environment. Select the appropriate testing to presumptively identify this organism. 1. motility 2. catalase 3. esculin hydrolysis 4. lipid requirement Question 109 The lab receives a blood culture from a veterinarian who has been ill for many weeks with fevers only in the a³ernoon and evenings, arthri±s and fa±gue. The blood culture is posi±ve a³er 5 days incuba±on and the organism has the following characteris±cs: Gram stain: small, GNB SBA: growth a±er 48hr. with small, smooth, raised colonies MAC: No growth present What should the microbiologist do next? Question 110 The serotype of O157:H7 of which of the following bacterial species is associated with a serious diarrheal illness with high mortality rates among some popula±ons? Question 111 Two patients, with a history of pharyngitis, are examined by their physician. One individual has symptoms characteristic of arthritis and heart disease. The other has symptoms characteristic of renal disease. Both of their original throat cultures grew beta hemolytic streptococci that were susceptible to Bacitracin. The diseases these two individuals are experiencing are probably: Question 112 What cellular component is NOT quantified in a smear from a sputum sample? Question 113 What color do Escherichia coli colonies appear on MacConkey agar? 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 20/24 Question 114 What color would a Positive Lysine decarboxylase tube turn? Question 115 What do the following list of organisms all have in common? Eikenella corrodens, Kingella kingae, Haemophilus aphrophilus , and Cardiobacterium hominis Question 116 What growth factors does H. inﬂuenzae type b require? Question 117 What ingredient MUST be present in the agar in order to perform an Indole test? Question 118 What is the ideal tes±ng method for detec±on of C. di±cile in a liquid stool? Question 119 What presump±ve tes±ng results indicate Pasteurella multocida ? Question 120 What test could a microbiologist perform to determine if a GNB was a glucose fermenter? Question 121 0 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 21/24 What tes±ng method (s) is/are ideal for diagnosis of Lyme disease if no erythema migrans is present? Question 122 When observing a gram stain from a sputum sample, the technologist noted purple nuclei in the WBCs. What does this indicate? Question 123 When you observe a clot in the Coagulase tube, you record this reaction as: Question 124 Which HACEK bacteria is o³en the cause of infec±on in human bite wounds or clenched-fist wounds? Question 125 Which fas±dious gram nega±ve bacillus is considered a poten±al bioterror weapon and the e±ologic agent of rabbit fever? Question 126 Which of following specimens is an error in the pre-analytical phase of laboratory testing? Question 127 Which of the following TSI reactions indicatesthe organism ferments glucose? Question 128 Which of the following antibiotics disks can be used to differentiate S. saprophyticus from other Coagulase-negative Staphylococcus sp. 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 22/24 Question 129 Which of the following genera is among the least biochemically reac±ve members of the Enterobacteriaceae? Question 130 Which of the following individuals is least likely to be colonized or infected with Pseudomonas aeruginosa ? Question 131 Which of the following is most likely to cause meningi±s in a child due to progression of a local infec±on? Question 132 Which of the following is the most important ingredient required for growth of F. tularensis? Question 133 Which of the following is the most sensi±ve test for B. pertussis iden±fica±on? Question 134 Which of the following media is di´erential but not selective? Question 135 Which of the following reactions characterize the genera Staphylococcus sp. and Micrococcus sp.? 1) Catalase negative 2) Catalase positive 3) Gram-positive cocci 4) Coagulase positive Question 136 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 0 out of 1 points 0 out of 1 points 1 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 23/24 Which organisms could be used for quality control tes±ng for the Indole test? Question 137 Which other nonfermenter shares a posi±ve lysine decarboxylase result with Burkholderia cepacia ? Question 138 Which specimen type takes the highest priority and would be set up first without delay? Question 139 Which specimens would be rejected due to improper specimen type collected for the type of culture ordered? Question 140 Which sugar is added to TSI agar and not found in KIA agar? Question 141 You observe the following colony morphologies on a specimen you are working up. What tests would you select for definitive identification? 1) 3+ Translucent, tiny, wide zone of beta hemolysis, GPC Question 142 You observe the following colony morphologies on a specimen you are working up. What tests would you select for definitive identification? 1 out of 1 points 1 out of 1 points 1 out of 1 points 1 out of 1 points 0 out of 1 points 0 out of 1 points

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5/3/2021 Review Test Submission: Final Exam Review Questions – (... https://blackboard.calhoun.edu/webapps/assessment/review/review.jsp?attempt_id=_10879433_1&course_id=_53639_1&content_id=_3673848_1&… 24/24 Monday, May 3, 2021 12:08:53 AM CDT 1) 2+ Large creamy, butyrous, beta hemolytic, GPC Question 143 You observe the following colony morphology on a sputum culture you are working up. What tests would you select for definitive identification? 1) 4+ Umbilicate, translucent, mucoid, alpha hemolytic, lancet shaped GPDC Question 144 You observe the following reaction from a Decarboxylase test: Yellow base tube (Uninoculated Negative Control) Purple Arginine tube Purple Lysine tube Purple ornithine tube What is your interpretation? Question 145 A 42 year old male, recently returned from a business trip to India, has developed severe watery diarrhea. His physician suspects the gastroenteritis is caused by Vibrio cholerae. Select the most useful medium for isolation and di´erentiation of this organism from stool. ← OK 1 out of 1 points 1 out of 1 points 1 out of 1 points

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