HIV rapid Virtual Lab 1

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Calhoun Community College *

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MISC

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Biology

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May 24, 2024

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Josephine Sullivan MLT 132 Virtual Lab Activity: HIV testing with Alere (formerly Abbott) kit (25 points) Directions: Watch the training videos for use of the Alere HIV rapid test kit illustrating how to use for serum, plasma, whole blood, and quality control materials. Read the package insert from the kit. Respond to the questions below and submit your completed lab worksheet through the Blackboard Assignment Drop Box submission link. Questions: 2 points each 1. List the 3 items this test is able to detect. a. HIV -1 antibodies b. HIV -2 antibodies c. HIV-1/2 p24 antigen 2. List acceptable patient specimens for use with this kit. Include acceptable anticoagulants. a. Venipuncture whole blood i. Serum (red top/tiger top with no anticoagulant) ii. Plasma (purple top with EDTA) b. Capillary blood whole blood 3. What reagents are included in the kit? a. HIV-1 p24 Antigen Control b. HIV-1 Reactive Control c. HIV-2 Reactive Control d. Nonreactive Control 4. When should EXTERNAL quality control samples be run? a. By a new or untrained person prior to patient testing b. When new lot is to be used or is received c. New shipment of test kit received d. Periodic intermittent control test as determined by facility 5. How much sample is applied to the sample pad? a. 50 microliters 6. If using the whole blood sampling method (capillary tube), why is it important to transfer all blood from the tube before lifting from the sample pad? a. To prevent air/bubbles from entering test pad

7. How long is the test incubated or timed for before reading results? a. Read after/between 20 to 30 minutes after chase buffer added to test area 8. What color line(s) appear for positive test results? a. Pink or red line for control/antibody/antigen lines (all three) b. Pink/red control and antibody lines (two lines) c. Pink/red control line and antigen line (two lines) 9. Is an INTERNAL quality control built into this test? a. Yes 10. In what conditions/diseases may a false positive test occur? See Limitations section of package insert. a. Individuals with Toxoplasma IgG, human anti-mouse antibodies, rheumatoid factor, elevated triglycerides, herpes simplex virus infection b. Hospitalized and cancer patients 11. If you have a line or lines in the test areas but no control line appears at the end of the incubation time, how is the result reported? a. Invalid test must be repeated Critical Thinking Questions: 12. What is meant by “preliminary positive” result with this test kit? What action is taken when the test is “preliminary positive?” See Limitations Section of package insert . (3 points) Preliminary positive means that the antigen or antibodies were detected and the sample is then sent out for confirmation quantitative test in lab

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