Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 9.4, Problem 2CC

WHAT IF? Ø In the absence of O2 as in question 1, what do you think would happen if you decreased the pH of the intermembrane space of the mitochondrion? Explain your answer.

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Compare the abundance of cristae in the mitochondria in Fig. 18.11b (677) with those in Fig. 20.21 (760). Is there a difference? What might this reflect in functional terms?
If isolated mitochondria are incubated with asource of electrons such as succinate, but without oxygen,electrons enter the respiratory chain, reducing each of theelectron carriers almost completely. When oxygen is thenintroduced, the carriers become oxidized at different rates(Figure Q14–1). How does this result allow you to order the electron carriers in the respiratory chain? What is theirorder?
You are isolating  mitochondria from insect cells and incubating in a test tube with 0.005 M FADH2, 0.05 M ADP and 0.05 M Pi. Assuming, these 3 can enter mitochondria at no cost,no glucose/products of glucose metabolism remain in the isolated mitochondria, and oxygen is present.  Part 1) If all expected reactions go to completion, how much ATP is expected formed?  (0 M)(0.02 M) (0.03 M) (0.05 M) (0.1 M) (0.005 M) (0.01 M) (0.015 M)  Part 2) The ratio of FADH2/FAD at completion of all expected reactions would be? (2) (>>2) (0) (1)  Part 3) After completion of all expected reactions, ratio of H ion concentration inside vs. outside the mitochondrial inner membrane should be?  (<1) (~1) (>1)(0)  Please provide brief explanation

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