Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Textbook Question
Chapter 9.1, Problem 3AYP
Compare the action of microbicidal and microbistatic agents.
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Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 9 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 9.1 - Clearly define the terms sterilization,...Ch. 9.1 - Prob. 2AYPCh. 9.1 - Compare the action of microbicidal and...Ch. 9.1 - Name four categories of cellular targets for...Ch. 9.2 - Prob. 5AYPCh. 9.2 - Prob. 6AYPCh. 9.2 - Prob. 7AYPCh. 9.2 - Prob. 8AYPCh. 9.2 - Prob. 9AYPCh. 9.2 - Prob. 10AYP
Ch. 9.2 - Differentiate between the two types of radiation...Ch. 9.2 - Prob. 12AYPCh. 9.3 - Name the desirable characteristics of chemical...Ch. 9.3 - Prob. 14AYPCh. 9.3 - List advantages and disadvantages to phenolic...Ch. 9.3 - Prob. 16AYPCh. 9.3 - Prob. 17AYPCh. 9.3 - Identify some heavy metal control agents.Ch. 9.3 - Prob. 19AYPCh. 9.3 - Identify applications for ethylene oxide...Ch. 9.3 - Prob. 1MMCh. 9.3 - Prob. 2NPCh. 9.3 - Q. What is the mode of action of chlorhexidine?Ch. 9.3 - In the health care environment, use of...Ch. 9 - Prob. 1NPCh. 9 - Prob. 1QCh. 9 - Prob. 2QCh. 9 - Triclosan and other antimicrobial compounds have...Ch. 9 - Microbial control methods that kill _____ are able...Ch. 9 - Prob. 5QCh. 9 - Prob. 6QCh. 9 - Prob. 7QCh. 9 - Prob. 8QCh. 9 - Prob. 9QCh. 9 - Prob. 10QCh. 9 - Do you think UV radiation is a good way to...Ch. 9 - Suggest a possible mechanism for how a microbe...Ch. 9 - Prob. 13QCh. 9 - Prob. 14QCh. 9 - What additional considerations would you have to...Ch. 9 - Sanitization is a process by which a. the...Ch. 9 - Prob. 17QCh. 9 - Defend this statement: It is possible to make our...Ch. 9 - The most versatile method for sterilizing...Ch. 9 - Precisely what is microbial death?Ch. 9 - Prob. 21QCh. 9 - From chapter 3, figure 3.15. Study this...
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