BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 9, Problem 7P
Interpretation Introduction
Interpretation:
If the addition of one gene encoding a restriction endonuclease by horizontal gene transfer is beneficial or not is to be stated.
Concept introduction:
The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the particular DNA sequence.
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From the reaction data below, determine whether the reaction is first order or second order and calculate the rate constant.
Time (s)
0
Reactant (mM)
5.4
1
4.6
2
3.9
3
3.2
4
2.7
5
2.3
Only a plot of In[reactant] versus t gives a straight line, so the reaction is
s-1.
. The negative of the slope, k, is
A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gel
electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecular masses of
180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol (DTT), three bands again
form, this time with molecular masses of 160, 90, and 60 kDa.
How many subunits does the protein have, and what is the molecular mass of each?
four subunits: 180, 160, 90, and 60 kDa
three subunits: 180, 160, and 60 kDa
three subunits: 160, 90, and 60 kDa
four subunits: 160, 90, 90, and 60 kDa
Correct Answer
Calculate KM and Vmax from the following data:
KM= i
Vmax
[S] (μM)
vo (mM.s-¹)
0.1
0.34
0.2
0.53
0.4
0.74
0.8
0.91
1.6
1.04
μM
mM s-1
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