An explanation corresponding to the fact that the mutation of the proton-shuttle residue His 64 to Ala in carbonic anhydrase II reduce the catalytic rate but in buffers with small molecular components, no reduction was observed, whereas in buffers with larger components, significant reduction were observed is to be stated. Concept introduction: Mutation is defined as the change in the sequence/order of DNA bases. It occurs due to the introduction of radiation, chemicals, or an internal disorder. Mutation may be caused by a single change in base sequence.

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Chapter 9, Problem 5P

Interpretation Introduction

Interpretation:

An explanation corresponding to the fact that the mutation of the proton-shuttle residue His 64 to Ala in carbonic anhydrase II reduce the catalytic rate but in buffers with small molecular components, no reduction was observed, whereas in buffers with larger components, significant reduction were observed is to be stated.

Concept introduction:

Mutation is defined as the change in the sequence/order of DNA bases. It occurs due to the introduction of radiation, chemicals, or an internal disorder. Mutation may be caused by a single change in base sequence.

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