BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<
BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<
8th Edition
ISBN: 9781305760738
Author: Campbell
Publisher: CENGAGE C
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Chapter 9, Problem 52RE
Interpretation Introduction

Interpretation:

The reason behind 50S ribosomal subunit and a 30S ribosomal subunit combine to form 70S subunit, instead of an 80S subunit is to be interpretated.

Concept Introduction:

Ribosomes and ribosomal RNA subunits has been studied on the basis of sedimentation coefficients. S is the Svedberg's unit. The sedimentation rate for a particle of a given size and shape measures how fast the particle 'settles'. The S value increase with the molecular weight of the sedimenting particle, but it is not directly proportional to it because the particle’s shape also affects its sedimentation rate.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?

Chapter 9 Solutions

BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<

Ch. 9 - REFLECT AND APPLY Why is RNA more vulnerable to...Ch. 9 - Prob. 12RECh. 9 - RECALL Draw a GC base pair. Draw an AT base pair.Ch. 9 - RECALL Which of the following statements is (are)...Ch. 9 - Prob. 15RECh. 9 - BIOCHEMICAL CONNECTIONS Describe the landmark case...Ch. 9 - Prob. 17RECh. 9 - Prob. 18RECh. 9 - RECALL Which of the following statements is (are)...Ch. 9 - RECALL Define supercoiling, positive supercoil,...Ch. 9 - RECALL What is propeller twist?Ch. 9 - RECALL What is an AG/CT step?Ch. 9 - RECALL Why does propeller-twist occur?Ch. 9 - Prob. 24RECh. 9 - RECALL If circular B-DNA is positively...Ch. 9 - RECALL Briefly describe the structure of...Ch. 9 - Prob. 27RECh. 9 - REFLECT AND APPLY List three mechanisms that relax...Ch. 9 - REFLECT AND APPLY Explain how DNA gyrase works.Ch. 9 - Prob. 30RECh. 9 - REFLECT AND APPLY Would you expect to find...Ch. 9 - REFLECT AND APPLY One of the original structures...Ch. 9 - REFLECT AND APPLY What is the complete base...Ch. 9 - REFLECT AND APPLY Why was it necessary to specify...Ch. 9 - Prob. 35RECh. 9 - Prob. 36RECh. 9 - Prob. 37RECh. 9 - BIOCHEMICAL CONNECTIONS A recent commercial for a...Ch. 9 - REFLECT AND APPLY A technology called PCR is used...Ch. 9 - REFLECT AND APPLY Why does DNA with a high AT...Ch. 9 - Prob. 41RECh. 9 - Prob. 42RECh. 9 - Prob. 43RECh. 9 - Prob. 44RECh. 9 - 45. Why does the absorbance increase when a DNA...Ch. 9 - Prob. 46RECh. 9 - REFLECT AND APPLY Would you expect tRNA or mRNA to...Ch. 9 - REFLECT AND APPLY The structures of tRNAs contain...Ch. 9 - REFLECT AND APPLY Would you expect mRNA or rRNA to...Ch. 9 - REFLECT AND APPLY Which would be more harmful to a...Ch. 9 - Explain briefly what happens to eukaryotic mRNA...Ch. 9 - Prob. 52RE
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