FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 9, Problem 26CQ
Summary Introduction

To explain: The difference between the archeal lipid that is given in the question and phosphatidylglycerol.

Concept introduction: Membrane lipids form the double-layered surface of cells. It is also called a lipid bilayer. Phospholipids, glycolipids, and cholesterol are the three main classes of the membrane lipids. The membrane lipids of the archaea are unique and distinct compared to the membrane lipids of Eukarya and Bacteria. Phosphatidylglycerol is an anionic lipid that is found in the mammalian membranes in the low amounts.

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A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin, chymotrypsin, and cyanogen bromide treatments are shown: ⚫ Trypsin digestion gave two fragments with multiple residues (not in order): • T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr o T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe • CT2: Thr, Trp • CT3: Lys, Met, Tyr, Val 。 CT4: Arg, Gly ⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between each residue.
A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin, chymotrypsin, and cyanogen bromide treatments are shown: • Trypsin digestion gave two fragments with multiple residues (not in order): T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr • T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CTI: Ala, Phe 。 CT2: Thr, Trp • CT3: Lys, Met, Tyr, Val 。 CT4: Arg, Gly ⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between each residue.
Poly-L-leucine in an organic solvent such as dioxane is a helical, whereas poly-L-isoleucine is not. Why do these amino acids with the same number and kinds of atoms have different helix-forming tendencies? L-isoleucine's methyl group sterically interferes with α-helix formation, whereas L-leucine's methyl group does not interfere. L-isoleucine lacks an NH group that can donate a hydrogen bond to stabilize an a helix, whereas L-leucine has an NH group. L-isoleucine contains a hydrogen-bond acceptor in close proximity to the main chain, whereas L-leucine lacks this group. L-isoleucine's ring structure prevents it from assuming the op value to fit into an a helix, whereas L-isoleucine does not have a ring structure.

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FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS

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