Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 9, Problem 11P
Interpretation Introduction

Interpretation:

The expected value for kcat at pH 6.0 is to be calculated.

Concept introduction:

The biological catalysts which lower the minimum amount of energy required by the reactants to convert into product are known as enzymes. An enzyme does not alter the potential energy of reactant and product. Carbonic anhydrase is an enzyme used in catalytic interconversion.

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Please help determine the standard curve for my Kinase Activity in Excel Spreadsheet. Link: https://mnscu-my.sharepoint.com/personal/vi2163ss_go_minnstate_edu/_layouts/15/Doc.aspx?sourcedoc=%7B958f5aee-aabd-45d7-9f7e-380002892ee0%7D&action=default&slrid=9b178ea1-b025-8000-6e3f-1cbfb0aaef90&originalPath=aHR0cHM6Ly9tbnNjdS1teS5zaGFyZXBvaW50LmNvbS86eDovZy9wZXJzb25hbC92aTIxNjNzc19nb19taW5uc3RhdGVfZWR1L0VlNWFqNVc5cXRkRm4zNDRBQUtKTHVBQldtcEtWSUdNVmtJMkoxQzl3dmtPVlE_cnRpbWU9eEE2X291ZHIzVWc&CID=e2126631-9922-4cc5-b5d3-54c7007a756f&_SRM=0:G:93 Determine the amount of VRK1 is present 1. Average the data and calculate the mean absorbance for each concentration/dilution (Please over look for Corrections) 2. Blank Correction à Subtract 0 ug/mL blank absorbance from all readings  (Please over look for Corrections) 3. Plot the Standard Curve  (Please over look for Corrections) 4. Convert VRK1 concentration from ug/mL to g/L  5. Use the molar mass of VRK1 to convert to M and uM…
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