Whether the given reduction in activity during the mutation of Ser 221 to Ala and His 64 to Ala is correct or not is to be identified. Concept introduction: Mutation is defined as the change in the sequence/order of DNA bases. It occurs due to the introduction of radiation, chemicals, or an internal disorder. Mutation may be caused by a single change in base sequence. Subtilisin refers to a protein-digesting enzyme. It initiates the attack of nucleophile on the amide bond by a serine residue.

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Chapter 9, Problem 3P

Interpretation Introduction

Interpretation:

Whether the given reduction in activity during the mutation of Ser 221 to Ala and His 64 to Ala is correct or not is to be identified.

Concept introduction:

Mutation is defined as the change in the sequence/order of DNA bases. It occurs due to the introduction of radiation, chemicals, or an internal disorder. Mutation may be caused by a single change in base sequence.

Subtilisin refers to a protein-digesting enzyme. It initiates the attack of nucleophile on the amide bond by a serine residue.

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