A.
To determine: What would happen if bacteria containing a phage in prophage state are irradiated with UV light and c1 protein is degraded.
Introduction: When bacteriophages infect a host cell, their DNA follows two fates in the form of lytic and lysogenic cycles. Lytic cycle refers to generation of new phages and lysis of a cell. Lysogeny also known as prophage stage refers to integration of phage in the host’s genome where it replicates with host DNA over many generations. These are controlled by c1 and Cro transcription regulators encoded by virus.
B.
To determine: Whether the change due to UV irradiation can be reversed when UV light is switched off.
Introduction: When bacteriophages infect a host cell, their DNA follows two fates in the form of lytic and lysogenic cycles. Lytic cycle refers to generation of new phages and lysis of a cell. Lysogeny also known as prophage stage refers to integration of phage in the host’s genome where it replicates with host DNA over many generations. These are controlled by c1 and Cro transcription regulators encoded by virus.
C.
To determine: Why this response to UV light might have evolved.
Introduction: When bacteriophages infect a host cell, their DNA follows two fates in the form of lytic and lysogenic cycles. Lytic cycle refers to generation of new phages and lysis of a cell. Lysogeny also known as prophage stage refers to integration of phage in the host’s genome where it replicates with host DNA over many generations. These are controlled by c1 and Cro transcription regulators encoded by virus.
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