Chapter 8, Problem 26P

Interpretation Introduction

(a)

Interpretation:

The rearrangement of the Michaelis-Menten equationfor obtaining the $V_0$ as a function of $V_0/\left[\text{S}\right]$ needs to be determined.

Concept introduction:

The Michaelis-Menten equation relates the concentration of substrate with the velocity of the reaction. A constant which expresses the substrate’s concentration if the velocity of reaction equals to the half of the maximum velocity of the reaction is known as Michaelis-Menten constant. It is denoted by $K_{\text{M}}$.

Interpretation Introduction

(b)

Interpretation:

The significance of the slope, the y-intercept, and the x -intercept in a plot of $V_0$ versus $V_0/\left[\text{S}\right]$ needs to be explained.

Concept introduction:

The Michaelis-Menten equation relates the concentration of substrate with the velocity of the reaction. A constant which expresses the substrate’s concentration if the velocity of reaction equals to the half of the maximum velocity of the reaction is known as Michaelis-Menten constant. It is denoted by $K_{\text{M}}$.

Interpretation Introduction

(c)

Interpretation:

The graph between $V_0$ and $V_0/\left[\text{S}\right]$ in inhibitor’s absence, in the presence of a competitive inhibitor and noncompetitive inhibitor needs to be plotted.

Concept introduction:

A molecule that disturbs or inhibits the activity of an enzyme is known as enzyme inhibitor. The enzyme inhibition stops the generation of enzyme-substrate complex which, in turn, inhibits the formation of the product. The three main types of inhibition are competitive inhibition, uncompetitive inhibition and noncompetitive inhibition.