Chapter 8, Problem 15P

Summary Introduction

To determine: The effect on sequencing process if the ${\text{3}}^{\prime }$–end- blocking group of each nucleotide were replaced with replaced with ${\text{3}}^{\prime }$–H present in dideoxynucleotides used in Sanger sequencing.

Introduction:

The determination of long DNA strands can be done by methods like Sanger sequencing. Sanger method is used for DNA sequencing in which a small concentration of dideoxynucleoside triphosphate $\text{ddCTP}$ is added along with $\text{dCTP}$.