Chapter 8, Problem 11P

Summary Introduction

To determine: The protein concentration in a solution of ${\text{A}}_{\text{280}}=\text{0}\text{.69}$ and ${\text{A}}_{\text{260}}=\text{0}\text{.94}$.

Introduction:

The protein concentration present in a solution of nucleic acid along with proteins can be determined by estimating their light absorption properties like protein absorb at the range of 280 nm and the range at which nucleic acid absorbs is 260 nm. Their respective concentration can be measured by the absorbance (A) of solutions at 260 and 280 nm.