Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 7.8, Problem 1MI
Why is it important to have no more than about 250 colonies on the plate when counting colonies to determine
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 7 Solutions
Prescott's Microbiology
Ch. 7.1 - MICRO INQUIRY In addition to chromosomes, what...Ch. 7.2 - MICRO INQUIRY Why is it important that the origin...Ch. 7.2 - MICRO INQUIRY What would be the outcome if FtsZ...Ch. 7.2 - MICRO INQUIRY Which step in the development of...Ch. 7.2 - Retrieve, Infer, Apply 1. Describe the three...Ch. 7.2 - Retrieve, Infer, Apply 2. How does the bacterial...Ch. 7.2 - Retrieve, Infer, Apply 3. Do you think MinCDE...Ch. 7.2 - Retrieve, Infer, Apply 4. Do you think Spiroplasma...Ch. 7.3 - What elements of the Sulfolobus spp. cell cycle...Ch. 7.3 - Many archaea have genes encoding an FtsZ...
Ch. 7.4 - MICRO INQUIRY Identify the regions of the growth...Ch. 7.4 - Retrieve, Infer, Apply Define microbial growth.Ch. 7.4 - Retrieve, Infer, Apply Describe the phases of the...Ch. 7.4 - Why would cells that are vigorously growing when...Ch. 7.4 - Calculate the growth rate constant and generation...Ch. 7.4 - Suppose the generation time of a bacterium is 90...Ch. 7.5 - What is the difference between halophilic and...Ch. 7.5 - Why do facultative anaerobes grow best at the...Ch. 7.5 - Retrieve, Infer, Apply 1. How do microorganisms...Ch. 7.5 - Retrieve, Infer, Apply 2. Define water activity...Ch. 7.5 - Prob. 1.3CCCh. 7.5 - Retrieve, Infer, Apply 1. Define pH, acidophile,...Ch. 7.5 - Retrieve, Infer, Apply Classify each of the...Ch. 7.5 - Retrieve, Infer, Apply 3. Describe the mechanisms...Ch. 7.5 - Retrieve, Infer, Apply 1. What are cardinal...Ch. 7.5 - Prob. 3.2CCCh. 7.5 - Prob. 3.3CCCh. 7.5 - Prob. 3.4CCCh. 7.5 - Retrieve, Infer, Apply Describe the five types of...Ch. 7.5 - Retrieve, Infer, Apply What are the toxic effects...Ch. 7.5 - Retrieve, Infer, Apply Where would you expect to...Ch. 7.5 - Retrieve, Infer, Apply List the types of...Ch. 7.5 - Prob. 5.3CCCh. 7.5 - Prob. 5.4CCCh. 7.6 - MICRO INQUIRY What biomolecules make up the...Ch. 7.6 - Prob. 1CCCh. 7.6 - Prob. 2CCCh. 7.6 - Prob. 3CCCh. 7.6 - Retrieve, Infer, Apply What is quorum sensing?...Ch. 7.6 - Retrieve, Infer, Apply How is the communication...Ch. 7.7 - Prob. 1CCCh. 7.7 - What are peptones, yeast extract, beef extract,...Ch. 7.7 - Retrieve, Infer, Apply Describe four ways in which...Ch. 7.7 - What are pure cultures and why are they important?...Ch. 7.7 - Retrieve, Infer, Apply It is known that microbial...Ch. 7.7 - Retrieve, Infer, Apply How might an enrichment...Ch. 7.8 - Why is it important to have no more than about 250...Ch. 7.8 - Briefly describe each technique by which microbial...Ch. 7.8 - Prob. 2CCCh. 7.8 - Prob. 3CCCh. 7.8 - For each of the following, which enumeration...Ch. 7.9 - Prob. 1MICh. 7.9 - Prob. 1CCCh. 7.9 - Prob. 2CCCh. 7.9 - Prob. 3CCCh. 7 - Prob. 1RCCh. 7 - Prob. 2RCCh. 7 - Prob. 3RCCh. 7 - Prob. 4RCCh. 7 - Prob. 5RCCh. 7 - Prob. 6RCCh. 7 - As an alternative to diffusible signals, suggest...Ch. 7 - If you wished to obtain a pure culture of bacteria...Ch. 7 - Prob. 3ALCh. 7 - Suggest one specific mechanism underlying the...Ch. 7 - Consider cell-cell communication: bacteria that...Ch. 7 - Suppose you discovered a new bacterial strain from...Ch. 7 - Prob. 7ALCh. 7 - Prob. 8ALCh. 7 - Prob. 9AL
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