Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 7.7, Problem 3RIA
Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter
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A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109.
a) How long was the generation time in minutes?b) How many generations have occurred?
Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution?
helpful formula:
g (generation time) = 0.301 (time)/ log x – log xo
What is the advantage of the plating method over an electronic cell counting method in counting cells?
Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?
Chapter 7 Solutions
Prescott's Microbiology
Ch. 7.1 - MICRO INQUIRY In addition to chromosomes, what...Ch. 7.2 - MICRO INQUIRY Why is it important that the origin...Ch. 7.2 - MICRO INQUIRY What would be the outcome if FtsZ...Ch. 7.2 - MICRO INQUIRY Which step in the development of...Ch. 7.2 - Retrieve, Infer, Apply 1. Describe the three...Ch. 7.2 - Retrieve, Infer, Apply 2. How does the bacterial...Ch. 7.2 - Retrieve, Infer, Apply 3. Do you think MinCDE...Ch. 7.2 - Retrieve, Infer, Apply 4. Do you think Spiroplasma...Ch. 7.3 - What elements of the Sulfolobus spp. cell cycle...Ch. 7.3 - Many archaea have genes encoding an FtsZ...
Ch. 7.4 - What is the difference between halophilic and...Ch. 7.4 - Why do facultative anaerobes grow best at the...Ch. 7.4 - Retrieve, Infer, Apply 1. How do microorganisms...Ch. 7.4 - Retrieve, Infer, Apply 2. Define water activity...Ch. 7.4 - What are halophiles and why do Halobacterium spp....Ch. 7.4 - Retrieve, Infer, Apply 1. Define pH, acidophile,...Ch. 7.4 - Retrieve, Infer, Apply Classify each of the...Ch. 7.4 - Retrieve, Infer, Apply 3. Describe the mechanisms...Ch. 7.4 - Retrieve, Infer, Apply 1. What are cardinal...Ch. 7.4 - Prob. 3.2RIACh. 7.4 - Define psychrophile, psychrotolerant, mesophile,...Ch. 7.4 - Retrieve, Infer, Apply What metabolic and...Ch. 7.4 - Retrieve, Infer, Apply Describe the five types of...Ch. 7.4 - Retrieve, Infer, Apply What are the toxic effects...Ch. 7.4 - Retrieve, Infer, Apply Where would you expect to...Ch. 7.4 - Retrieve, Infer, Apply List the types of...Ch. 7.4 - Prob. 5.3RIACh. 7.4 - Prob. 5.4RIACh. 7.5 - MICRO INQUIRY What biomolecules make up the...Ch. 7.5 - Prob. 1RIACh. 7.5 - Prob. 2RIACh. 7.5 - Retrieve, Infer, Apply What is quorum sensing?...Ch. 7.5 - Retrieve, Infer, Apply How is the communication...Ch. 7.6 - Prob. 1RIACh. 7.6 - What are peptones, yeast extract, beef extract,...Ch. 7.6 - Retrieve, Infer, Apply Describe four ways in which...Ch. 7.6 - What are pure cultures and why are they important?...Ch. 7.6 - Retrieve, Infer, Apply It is known that microbial...Ch. 7.6 - Retrieve, Infer, Apply How might an enrichment...Ch. 7.7 - MICRO INQUIRY Identify the regions of the growth...Ch. 7.7 - Retrieve, Infer, Apply Define microbial growth.Ch. 7.7 - Retrieve, Infer, Apply Describe the phases of the...Ch. 7.7 - Why would cells that are vigorously growing when...Ch. 7.7 - Retrieve, Infer, Apply Contrast and compare the...Ch. 7.7 - Calculate the growth rate constant and generation...Ch. 7.7 - Suppose the generation time of a bacterium is 90...Ch. 7.8 - Why is it important to have no more than about 250...Ch. 7.8 - Briefly describe each technique by which microbial...Ch. 7.8 - Prob. 2RIACh. 7.8 - Prob. 3RIACh. 7.8 - For each of the following, which enumeration...Ch. 7.9 - Prob. 1MICh. 7.9 - Prob. 1RIACh. 7.9 - Prob. 2RIACh. 7.9 - Prob. 3RIACh. 7 - As an alternative to diffusible signals, suggest...Ch. 7 - If you wished to obtain a pure culture of bacteria...Ch. 7 - Design an experiment to determine if a...Ch. 7 - Suggest one specific mechanism underlying the...Ch. 7 - Consider cell-cell communication: bacteria that...Ch. 7 - Suppose you discovered a new bacterial strain from...Ch. 7 - Because many persistent bacterial infections...
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- You have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?arrow_forwardThere are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyarrow_forwardIf a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it take to get 1,048,576 cells?arrow_forward
- How long does it take for a bacterial culture with a generation time of 12 minutes to increase from 400 cells up to 1600 cells/mL?arrow_forwardYou just finished plating your electroporatio products on your YPD-Zeocin plate and you think you did everything perfectly but you come back the folloeing week and have zero colonies. Which of the following could be the reason for the absence of colonies? A) You centrifuged your electroporated cells for 30 sec at 16,000 rpm instead of 4,000 rpm before plating them B) You added sorbitol+YPD to your cells thirty minutes after pulsing your cells C) The Zeocin had not been added to the plates when they were made. D) All of the above E) Both a and b onlyarrow_forwardOn agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?arrow_forward
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?arrow_forwardGiven a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?arrow_forwardYou found a bright blue growth on a tomato you had left on your kitchen counter. You’ve never seen a microorganism that color, and you want to know what it is. You take the tomato into the lab, sterilize an inoculating loop, transfer a sample from the tomato to a Nutrient Agar Petri dish, and incubate it at 37°C. But when you come back two days later, your Petri dish is empty. Why might this be? Be specific about what might have happened. How would you test your hypothesis?arrow_forward
- You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?arrow_forwardWhy is it important to use a sterilized loop between streaks when preparing a streakplate? Observation of a streakplate culture shows more growth in Quadrant 4 than in Quadrant 3. Account for this observation. Describe the way in which you can isolate an individual colony from a spreadplate or a streakplate that holds multiple colonies. Outline the differences between a streak plate and a spread plate.arrow_forwardTaking microbial cells out of a stationary phase culture and putting them into a fresh, sterile medium (in which they can grow) will result in the cells entering which stage of the growth cycle? Death phase O Log phase Lag phase Stationary phasearrow_forward
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