The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram. Given: The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below: Table 1: Labeled proteins observed at different intervals after the incubation of 32 P-EnvZ with OmpR. Duration after mixing 32 P-EnvZ and OmpR (min) 0 2 5 10 Presence of 32 P label in EnvZ High Medium Low Absent Presence of 32 P label in OmpR Absent Low Medium High Introduction: Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Question

Want to see the full answer?

Answer 1

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Biology You’re going to analyze 5 ul of your PCR product(out of 50 ul) on the gel. How much of 6X DNAloading buffer (dye) are you going to mix with yourPCR product to make final 1X concentration ofloading buffer in the PCR product-loading buffermixture?

Write the assignment on the title "GYMNOSPERMS" focus on the explanation of its important families, characters and reproduction.

Awnser these Discussion Questions Answer these discussion questions and submit them as part of your lab report. Part A: The Effect of Temperature on Enzyme Activity Graph the volume of oxygen produced against the temperature of the solution. How is the oxygen production in 30 seconds related to the rate of the reaction? At what temperature is the rate of reaction the highest? Lowest? Explain. Why might the enzyme activity decrease at very high temperatures? Why might a high fever be dangerous to humans? What is the optimal temperature for enzymes in the human body? Part B: The Effect of pH on Enzyme Activity Graph the volume of oxygen produced against the pH of the solution. At what pH is the rate of reaction the highest? Lowest? Explain. Why does changing the pH affect the enzyme activity? Research the enzyme catalase. What is its function in the human body? What is the optimal pH for the following enzymes found in the human body? Explain. (catalase, lipase (in your stomach),…

Answer 2

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Answer 6

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Answer 7

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 7.1 - Prob. 1R Ch. 7.1 - Prob. 2R Ch. 7.1 - Prob. 3R Ch. 7.2 - Prob. 1R Ch. 7.2 - Prob. 2R Ch. 7.2 - Prob. 3R Ch. 7.3 - Prob. 1R Ch. 7.3 - Prob. 2R Ch. 7.3 - Prob. 3R Ch. 7.4 - Prob. 1R

Solution Summary: The author describes the signal transduction pathway of Escherichia coli in response to a hypertonic solution.

Concept explainers

Videos

Want to see the full answer?

Chapter 7 Solutions

	Duration after mixing ³²P-EnvZ and OmpR (min)
	0	2	5	10
Presence of ³²P label in EnvZ	High	Medium	Low	Absent
Presence of ³²P label in OmpR	Absent	Low	Medium	High