
EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 7, Problem 3Q
Summary Introduction
To review:
Evidence of signal amplification in the signal transduction pathway.
Introduction:
Different genes of a cell get activated in different circumstances. Bacterial cells like Escherichia coli express a high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins, such as membrane protein (EnvZ), act as a protein kinase and get phosphorylated. They also phosphorylate proteins like OmpR (cytoplasm protein).
The amount of EnvZ and OmpR after incubation with 32P-radiolabeled ATP is recorded in the following table:
Table A
An isotonic solution consisting of nutrient broth only | A hypertonic solution consisting of Nutrient broth and 20% sucrose solution | |
EnvZ | 63 | 113 |
OmpR | 2043 | 3525 |
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