Chapter 7, Problem 3P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Separating Glycated Hb From Normal Hb (Integrates with Chapters 5 and 6.) Human hemoglobin can react with sugars in the blood (usually glucose) to form covalent adducts. The α-amino groups of N-terminal valine in the Hb β-subunits react with the C-1 (aldehyde) carbons of monosaceharides to form aldimine add wets, which rearrange to form very stable ketoamine products. Quantitation of this “glycated hemoglobin" is important clinically, especially for diabetic individuals. Suggest at least three methods by which glycated lib (also referred to as HbA1c) could be separated from normal Hb and quantitated.