EBK BIOLOGY
EBK BIOLOGY
5th Edition
ISBN: 8220100667978
Author: Maier
Publisher: PEARSON
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Chapter 7, Problem 3LTB
Summary Introduction

Introduction:

A gene is the basic functional unit for heredity. Genes are composed of the DNA strands and are located on the chromosomes. Genes are instructions to make the protein by the process of transcription and translation. The location where the gene is placed in the chromosome is termed as the locus of that gene.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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