Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 7, Problem 3CT
Human cells can be stained with crystal violet and safranin, so why can’t human cells be
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Different stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.
In lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative
bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the
counter-stain safranin).
a) What is the biological molecule that is present in these bacteria that produces different results
between Gram-positive and Gram-negative bacteria (be specific!) AND
b) What is the difference between Gram-positive and Gram-negative bacteria that creates the
different staining patterns (purple vs. pink)?
(2-3 sentences max)
Explain Why do acidic dyes not stain bacterial cells?
Chapter 7 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 7 - Why do gram-positive cells more than 24 hours old...Ch. 7 - Can iodine be added before the primary stain in a...Ch. 7 - Prob. 3QCh. 7 - Which step can you omit without affecting...Ch. 7 - Suppose you performed a Gram stain on a sample...Ch. 7 - Prob. 2CTCh. 7 - Human cells can be stained with crystal violet and...Ch. 7 - Considering that it isnt possible to identity...
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- 1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structuresarrow_forwardBoth crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardArchaea with cell walls consisting of a thick, homogeneous layer of complex polysaccharides often retain crystal violet dye when stained using the Gram-staining procedure. Suggest a reason for this staining reaction.arrow_forward
- Describe why Gram negative bacteria stain pink.arrow_forwardFor various species or portions of organisms, different stains have different affinities. Explain this assertion using the example of hematoxylin and eosin staining.arrow_forwarda. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?arrow_forward
- Why are bacterial cells generally stained for microscopic viewing?arrow_forwardWhy is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.arrow_forwardThe acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a gram stain is to be performed on this particular microbe, it often takes the gram stain reaction. Why?arrow_forward
- Why does UV light have to be directly exposed and waved around an object that needs to be disinfected and not waved around a bag with the items that need to be disinfected inside of it?arrow_forwardWhen doing a gram stain in microbiology, one step can be eliminated and still allow distinction between gram-positive and gram-negative organisms. What is the step and why would eliminating it still result in the ability to distinguish gram-positive from gram-negative?arrow_forwardAre the structural differences between the cell walls of Gram-positive and Gram-negative bacteria responsible in any way for the Gram stain reaction?arrow_forward
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