Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 7, Problem 1CA
Considering that it isn’t possible to identity bacteria horn a
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Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?
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Chapter 7 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 7 - Why do gram-positive cells more than 24 hours old...Ch. 7 - Can iodine be added before the primary stain in a...Ch. 7 - Prob. 3QCh. 7 - Which step can you omit without affecting...Ch. 7 - Suppose you performed a Gram stain on a sample...Ch. 7 - Prob. 2CTCh. 7 - Human cells can be stained with crystal violet and...Ch. 7 - Considering that it isnt possible to identity...
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- You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?arrow_forwardIn the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?arrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative Stainarrow_forward
- Define the term gram staining.arrow_forwardDifferentiate the staining procedures. Gram Stain Acid Fast Endospore Capsule Flagella a) Principle b) Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) c) Stain/s Used d) Type of Stain e) Mechanism of Staining (of each stain used) f) Flow of Methodarrow_forwardIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?arrow_forward
- N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00arrow_forwardWhich of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forwardWhat staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forward
- Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the procedure could cause an incorrect result?arrow_forwardFor a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?arrow_forwardWhy is oil immersion necessary to see bacteria in the laboratory?arrow_forward
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