Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 7, Problem 18P
List the order in which the following proteins and enzymes are active in E. coli
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Considering prokaryotes, what term adds nucleotides in the 5' to 3' direction during DNA replication?
For the following steps in DNA replication, name the prokaryotic and eukaryotic enzymes (in that order!) that perform the task listed:
Question 33 options:
Tethering a polymerase to DNA (prokaryotes)
Removing supercoils (eukaryotes)
Removing supercoils (prokaryotes)
Lagging strand replication (eukaryotes)
Lagging strand replication (prokaryotes)
Tethering a polymerase to DNA (eukaryotes)
Stabilizing single-stranded DNA (prokaryotes)
Stabilizing single-stranded DNA (eukaryotes)
1.
DNA Pol III
2.
DNA Pol Delta ()
3.
DNA Pol Epsilon ()
4.
Helicase
5.
Topoisomerase (type I or II)
6.
Beta clamp
7.
PCNA
8.
SSB
9.
RPA
10.
DNA Pol I
11.
Ligase
Define DNA replication/synthesis and semiconservative replication. In addition, describe and/or define the role(s) of each of the following in the process of DNA replication/synthesis: DNA template strand, 5’ and 3’ ends, DNA helicase, DNA polymerase, single-strand binding proteins, topoisomerase, primase, Okazaki fragments, leading strand and lagging strand.
Chapter 7 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 7 - What results from the experiments of Frederick...Ch. 7 - 7.2 Explain why Avery, MacLeod, and McCarty’s in...Ch. 7 - 7.3 Hershey and Chase selected the bacteriophage...Ch. 7 - 7.4 Explain how the Hershey and Chase experiment...Ch. 7 - 7.5 One strand of a fragment of duplex DNA has the...Ch. 7 - 7.6 The principles of complementary base pairing...Ch. 7 - For the following fragment of DNA, determine the...Ch. 7 - 7.8 Figures present simplified depictions of...Ch. 7 - 7.9 Consider the sequence -ACGCTACGTC-.
What is...Ch. 7 - DNA polymerase III is the main DNA-synthesizing...
Ch. 7 - There is a problem completing the replication of...Ch. 7 - Explain how RNA participates in DNA replication.Ch. 7 - A sample of double-stranded DNA is found to...Ch. 7 - Bacterial DNA polymerase I and DNA polymerase III...Ch. 7 - Diagram a replication fork in bacterial DNA and...Ch. 7 - Prob. 16PCh. 7 - Which of the following equalities is not true for...Ch. 7 - List the order in which the following proteins and...Ch. 7 - Two viral genomes are sequenced, and the following...Ch. 7 - Matthew Meselson and Franklin Stahl demonstrated...Ch. 7 - Raymond Rodriguez and colleagues demonstrated...Ch. 7 - 7.22 Joel Huberman and Arthur Riggs used pulse...Ch. 7 - 7.23 Why do the genomes of eukaryotes, such as...Ch. 7 - Bloom syndrome (OMIM 210900) is an autosomal...Ch. 7 - 7.25 How does rolling circle replication (see...Ch. 7 - Telomeres are found at the ends of eukaryotic...Ch. 7 - A family consisting of a mother (I-1), a father...Ch. 7 - In a dideoxy DNA sequencing experiment, four...Ch. 7 - Prob. 29PCh. 7 - Using an illustration style and labeling similar...Ch. 7 - A PCR reaction begins with one double-stranded...Ch. 7 - Prob. 32PCh. 7 - Prob. 33PCh. 7 - 7.34 A sufficient amount of a small DNA fragment...Ch. 7 - You are participating in a study group preparing...Ch. 7 - Prob. 36PCh. 7 - The following diagram shows the parental strands...Ch. 7 - Go to the OMIM website...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Summarize the functions of the following proteins in E. coli DNA replication: DNA polymerase I, DNA polymerase III, DnaA, helicase, SSB, primase, the sliding clamp, clamp loader, DNA ligase, Tus, and topoisomerases.arrow_forwardDNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MM-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MMR-minus and wildtype yeast? More than one answer is correct. (a) More point mutations will arise in MMR-minus yeast. Fewer point mutations will arise in MMR-minus yeast as compared with wildtype. (b) Of the total point mutations that do occur, the fraction in which G is replaced by C will be…arrow_forwardList and describe the sequential steps of DNA replication in prokaryotic (bacterial) cells. Be sure to include the key enzymes involved in this process.arrow_forward
- DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MER-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MER-minus and wildtype yeast? More than one answer is correct. Options: More point mutations will arise in MER-minus yeast. Fewer point mutations will arise in MER-minus yeast as compared with wildtype. Of the total point mutations that…arrow_forwardBelow is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers (1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA?arrow_forwardThe proteins and enzymes listed below are all required for DNA replication in E. coli, but they are listed in a random order. Determine the correct order in which they function in replication, by selecting the correct number from the drop-down menu in each case, with 1 being first and 6 being last.arrow_forward
- Discuss DNA replication of prokaryotes and please mention all of the enzymes and components listed below. DNA Primase – DNA directed “RNA Pol” which inserts nucleoside triphosphates (NTPs) Primers are oligonucleotides; priming process is the formation of primers DNA Helicase – separates the DNA in advance of the replication fork (in E. coli DNA Helicase II); binds at AT-rich region of DNA; ATP then binds the helicase Single-stranded DNA-binding Protein (SSBP) – no enzymatic activity; does not consume ATP Topoisomerase – alter the supercoiling of double-stranded DNA DNA Ligase – nicking of strands done for replication to continue Okazaki fragments DNA Polymerase – removes primer via 5’ to 3’ exonuclease activity; comes again for 5’ to 3’ polymerization activity (closes the gap between Okazaki fragments) Prokaryotes DNA Pol I – auxiliary enzyme to DNA Pol III; repairs damage; capable of excising pyrimidine dimers; polymerization via single active site that can bind all 4 dNTPs;…arrow_forwardThe Escherichia coli chromosome is a circular DNA molecule and contains a single origin of replication. List the mechanistic steps that can terminate the movement of replication forks in this chromosome.arrow_forwardThe figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helixarrow_forward
- Assume a deletion occurs in a gene that encodes DNA polymerase I and no functional DNA polymerase I is produced. What will be the most likely consequence of this mutation? The DNA would not exist in a supercoiled state. There would be no RNA primers laid down. The DNA will not be able to unwind to initiate replication. The DNA strands would contain pieces of RNA. There would be no DNA replication on the leading or lagging strands.arrow_forwardName of a few enzymes involved in DNA replication other than DSNA polymerase and ligase.name the key function each of them?arrow_forwardWhich of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true? Only NER involves the action of DNA ligase to seal nicks in the DNA backbone. Both NER and BER involve DNA strand cleavage by an endonuclease. Both NER and BER can be activated by exposure to visible light. Only BER requires DNA polymerase. Both NER and BER involve the creation of an apyrimidinic (AP) site.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Biology (MindTap Course List)BiologyISBN:9781305112100Author:Cecie Starr, Beverly McMillanPublisher:Cengage Learning
Human Biology (MindTap Course List)
Biology
ISBN:9781305112100
Author:Cecie Starr, Beverly McMillan
Publisher:Cengage Learning
Enzyme Kinetics; Author: MIT OpenCourseWare;https://www.youtube.com/watch?v=FXWZr3mscUo;License: Standard Youtube License