NESTER'S MICROBIOLOGY: A HUMAN PERSPECT
9th Edition
ISBN: 9781260161373
Author: Anderson, SALM
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Question
Chapter 7, Problem 10SA
Summary Introduction
To review:
The reasons for the difficulties encountered in locating the protein-coding regions in a genomic sequence.
Introduction:
The amino acids are the building blocks of proteins. Protein encoding is initiated when a stretch of DNA (deoxyribonucleic acid) encodes genetic information for the amino acid sequence specific for the future protein. This is followed by the transcription process, in which this genetic information is transferred to mRNA (messenger ribonucleic acid) from any of the two strands of the DNA. Once the mRNA is formed, it undergoes processing steps like splicing, tailing, and capping. This removes the introns or the noncoding sequences. Ultimately, the final step occurs in protein synthesis called translation. Locating anonymous protein-codingregionsin a genomic sequence can lead to ambiguity.
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Students have asked these similar questions
19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 7 Solutions
NESTER'S MICROBIOLOGY: A HUMAN PERSPECT
Ch. 7 - Explain what semiconservative means with respect...Ch. 7 -
2. What is an origin of replication?
Ch. 7 - Why are primers required in DNA replication but...Ch. 7 -
4. What is polycistronic mRNA?
Ch. 7 -
5. Explain why knowing the orientation of a...Ch. 7 - What is the function of a sigma factor?Ch. 7 - Prob. 7SACh. 7 - Explain how some bacteria sense the density of...Ch. 7 -
9. Compare and contrast regulation by a repressor...Ch. 7 - Prob. 10SA
Ch. 7 - All of the following are involved in transcription...Ch. 7 -
2. All of the following are involved in DNA...Ch. 7 - All of the following are directly involved in...Ch. 7 - Using the DNA strand shown here as a template,...Ch. 7 -
5. A ribosome binds to the following mRNA at the...Ch. 7 - Which of the following statements about gene...Ch. 7 -
7. An enzyme used to synthesize the amino acid...Ch. 7 -
8. Under which of the following conditions will...Ch. 7 -
9. All of the following are characteristics of...Ch. 7 - Which of the following statements is false? a) A...Ch. 7 - A graduate student is trying to identity the gene...Ch. 7 - To isolate eukaryotic mRNA from cell lysates, a...Ch. 7 - Prob. 1CTCh. 7 -
2. In a variation of the experiment in the...
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