Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
8th Edition
ISBN: 9780134015187
Author: John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher: PEARSON
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Chapter 6.2, Problem 6.7P
Interpretation Introduction
Interpretation:
The micromoles of Uric acid are contained in the stone should be determined.
Concept introduction:
Causes of kidney stone:
When your urine contains more crystal forming substance like calcium, oxalate and uric acid –than the fluid in our urine cam dilute.
At the same time, our urine may lack substance that prevent crystals from sticking together, creating an ideal environment for kidney stone to form
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 6 Solutions
Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
Ch. 6.1 - Calculate the molecular weight of the following...Ch. 6.1 - How many molecules of ascorbic acid (vitamin C,...Ch. 6.1 - What is the mass in grams of 5.0 1020 molecules...Ch. 6.1 - What is the molecular weight of cytosine, a...Ch. 6.2 - Prob. 6.5PCh. 6.2 - Prob. 6.6PCh. 6.2 - Prob. 6.7PCh. 6.3 - Prob. 6.8PCh. 6.3 - Prob. 6.9PCh. 6.4 - Hydrogen fluoride is one of the few substances...
Ch. 6.4 - The tungsten metal used for filaments in light...Ch. 6.5 - What is the theoretical yield of ethyl chloride in...Ch. 6.5 - The reaction of ethylene oxide with water to give...Ch. 6.5 - Prob. 6.14KCPCh. 6.5 - Dietary iron forms a 1:1 complex with hemoglobin...Ch. 6.5 - Prob. 6.2CIAPCh. 6.5 - Prob. 6.3CIAPCh. 6 - Methionine, an amino acid used by organisms to...Ch. 6 - Prob. 6.16UKCCh. 6 - Prob. 6.17UKCCh. 6 - Prob. 6.18UKCCh. 6 - Prob. 6.19UKCCh. 6 - Prob. 6.20APCh. 6 - What is the difference between molecular weight...Ch. 6 - Prob. 6.22APCh. 6 - Prob. 6.23APCh. 6 - How many calcium atoms are in 16.2 g of calcium?Ch. 6 - What is the mass in grams of 2.68 1022 atoms of...Ch. 6 - Calculate the molar mass of each of the following...Ch. 6 - Prob. 6.27APCh. 6 - Prob. 6.28APCh. 6 - Caffeine has the formula C8H10N4O2. If an average...Ch. 6 - How many moles of aspirin, C9H8O4, are in a 500 mg...Ch. 6 - What is the molar mass of diazepam (Valium),...Ch. 6 - Calculate the molar masses of the following...Ch. 6 - How many moles are present in a 4.50 g sample of...Ch. 6 - How many grams are present in a 0.075 mol sample...Ch. 6 - The principal component of many kidney stones is...Ch. 6 - Prob. 6.36APCh. 6 - Ethyl acetate reacts with H2 in the presence of a...Ch. 6 - Prob. 6.38APCh. 6 - Ammonia, NH3, is prepared for use as a fertilizer...Ch. 6 - Hydrazine, N2H4, a substance used as rocket fuel,...Ch. 6 - Prob. 6.41APCh. 6 - Magnesium metal burns in oxygen to form magnesium...Ch. 6 - Titanium metal is obtained from the mineral...Ch. 6 - Prob. 6.44APCh. 6 - Prob. 6.45APCh. 6 - Prob. 6.47APCh. 6 - Prob. 6.48APCh. 6 - Once made by heating wood in the absence of air,...Ch. 6 - In Problem 6.40, hydrazine reacted with oxygen...Ch. 6 - Dichloromethane, CH2Cl2, the solvent used to...Ch. 6 - Cisplatin [Pt(NH3)2Cl2], a compound used in cancer...Ch. 6 - Prob. 6.53APCh. 6 - Prob. 6.54APCh. 6 - Prob. 6.55CPCh. 6 - Prob. 6.56CPCh. 6 - Prob. 6.57CPCh. 6 - Prob. 6.58CPCh. 6 - Prob. 6.59CPCh. 6 - Prob. 6.60CPCh. 6 - Gaseous ammonia reacts with oxygen in the presence...Ch. 6 - Sodium hypochlorite, the primary component in...Ch. 6 - Barium sulfate is an insoluble ionic compound...Ch. 6 - The last step in the production of nitric acid is...Ch. 6 - Acetylsalicylic acid, the active ingredient in...Ch. 6 - Jewelry and tableware can be silver-plated by...Ch. 6 - Elemental phosphorus exists as molecules of P4. It...Ch. 6 - Lithium oxide is used aboard the International...Ch. 6 - Prob. 6.69CPCh. 6 - Prob. 6.70GPCh. 6 - Obtain a bottle of aspirin and identify the amount...Ch. 6 - Lovastatin, a drug used to lower serum...Ch. 6 - Pyrite, also known as fools gold, is used...
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