Microbiology: An Introduction, Books a la Carte Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition)
13th Edition
ISBN: 9780134729336
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 6, Problem 9R
DRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time of 30 minutes at 35°C, 60 minutes at 20°C, and 3 hours at 5°C.
- a. The cells are incubated for 5 hours at 35°C.
- b. After 5 hours, the temperature is changed to 20°C for 2 hours.
- c. After 5 hours at 35°C, the temperature is changed to 5°C for 2 hours followed by 35°C for 5 hours.
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
E.coli was incubated with aeration in a nutrient medium containing two carbon sources provided one at a time, and the following growth curve was made from this culture.
a. Explain what happened at the time marked x.
b. Which substrate provided “better” growth conditions for the bacteria? How can you tell?
Chapter 6 Solutions
Microbiology: An Introduction, Books a la Carte Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition)
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