MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
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Chapter 6, Problem 8RQ
Summary Introduction

To review:

The isolation of virus and its difference from bacterial isolation.

Introduction:

Virus are host dependent acellular organisms. They do not have active metabolism and possess minimal genes for replication, transcription, and translation. They hijack the host cell machinery to reproduce by either integrating their genome into the host cell genome or actively producing multiple virions and releasing them by lysis or budding off from the host cell.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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