Campbell Biology (10th Edition)
10th Edition
ISBN: 9780321775658
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 10TYU
SCIENTIFIC INQUIRY Imagine protein X, destined to span the plasma membrane. Assume that the mRNA carrying the genetic message for protein X has already been translated by ribosomes in a cell culture. If you fractionate the cells (see Figure 6.4), in which fraction would you find protein X? Explain by describing its transit through the cell.
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If you did a classic Pulse-Chase experiment to follow the path of a secreted protein, and you forgot to do the Chase, which of the following would you observe?
No radioactivity would be seen in any of the structures.
Radioactivity would increase first in rough ER, then Golgi Complex, then secretory vesicles, but it would remain high in all, never decreasing.
Nothing would change
Radioactivity would increase in all 3 structures simultaneously and stay high.
Describe, in detail, the steps involved in the synthesis of an integral membrane
protein located in the plasma membrane. Begin with the ribosome, end with the
plasma membrane.
Predict what the results would be if mRNA were radioactively labeled instead of polypeptides. Give one or two reasonings to support your prediction.
Chapter 6 Solutions
Campbell Biology (10th Edition)
Ch. 6.1 - Prob. 1CCCh. 6.1 - Prob. 2CCCh. 6.2 - Briefly describe the structure and function of the...Ch. 6.2 - Prob. 2CCCh. 6.3 - What role do ribosomes play in carrying out...Ch. 6.3 - Describe the molecular composition of nucleoli and...Ch. 6.3 - Prob. 3CCCh. 6.4 - Describe the structural and functional...Ch. 6.4 - Describe how transport vesicles integrate the...Ch. 6.4 - WHAT IF? Imagine a protein that functions in the...
Ch. 6.5 - Describe two characteristics shared by...Ch. 6.5 - Prob. 2CCCh. 6.5 - Prob. 3CCCh. 6.6 - Prob. 1CCCh. 6.6 - WHAT IF? Males afflicted with Kartagener's...Ch. 6.7 - In what way are the cells of plants and animals...Ch. 6.7 - Prob. 2CCCh. 6.7 - MAKE CONNECTIONS The polypeptide chain that makes...Ch. 6 - Prob. 6.1CRCh. 6 - Explain how the compartmental organization of a...Ch. 6 - Describe the relationship between the nucleus and...Ch. 6 - Describe the key role played by transport vesicles...Ch. 6 - Prob. 6.5CRCh. 6 - Describe the role of motor proteins inside the...Ch. 6 - Prob. 6.7CRCh. 6 - Which structure is not part of the endomembrane...Ch. 6 - Prob. 2TYUCh. 6 - Which of the following is present in a prokaryotic...Ch. 6 - Prob. 4TYUCh. 6 - Prob. 5TYUCh. 6 - Prob. 6TYUCh. 6 - Prob. 7TYUCh. 6 - Prob. 8TYUCh. 6 - EVOLUTION CONNECTION (a) What cell structures best...Ch. 6 - SCIENTIFIC INQUIRY Imagine protein X, destined to...Ch. 6 - WRITE ABOUT A THEME: ORGANIZATION Considering some...Ch. 6 - SYNTHESIZE YOUR KNOWLEDGE The cells in this SEM...
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- Free ribosomes in the cytosol and ribosomes bound to the ER membrane have: 1) different types of small subunits but the same large subunits 2) the same types of large and small subunits 3) different types of large and small subunits 4) different types of large subunits the same small subunitsarrow_forwardChoose the correct order of events for protein synthesis of an INTEGRAL MEMBRANE PROTEIN. 1. Translation occurs on a ribosome in the cytosol 2. Vesicle containing protein fuses with the Golgi apparatus. 3. Vesicle containing protein buds off the rough endoplasmic reticulum. 4. Vesicle containing protein fuses with the plasma membrane. 5. Transcription leads to generation of the MRNA. 6. Vesicle containing protein fuses with the rough endoplasmic reticulum. 5,1,6,4 5,3,2,4 2,6,3,4 1,6,2,4arrow_forwardSummarize the processing of a protein from the time a ribosome finishes its work to the time a protein is secreted from the cell. What roles do the endoplasmic reticulum and Golgi complex play in this?arrow_forward
- The figure above shows the organization of a protein that will eventually reside in the plasma membrane. The boxes labeled 1 and 2 represent membrane-spanning sequences and the arrow represents a site of action of signal peptidase. Which of the following statements must be TRUE about this protein? O a) Most of this protein will project from the cytosolic side of the membrane. O b) The mature version of this protein will span the membrane twice. c) This is a multi-pass membrane protein. O d) The mature version of this protein will span the membrane once.arrow_forwardWhat is the primary characteristic of peripheral membrane proteins? A: They cannot be post-translationally modified. B: They span the entire lipid bilayer, sometimes multiple times. C: Detergents or nonpolar solvents are necessary to remove them from the lipid bilayer. D:Weak intermolecular forces keep them attached to the lipid membrane.arrow_forwardImagine a protein which functions in ER but requires modification in the Golgi apparatus before it can achieve that function. Describe the protein’s path through the cell starting with protein in ER, with the help of diagram.arrow_forward
- Using the the enzyme acid hydrolase in the lysosome: What is the final destination in which the protein will function? Which features will the protein receive during its manufacture? What is the primary structure (general)? Where is the primary structure made? Where are the secondary and tertiary structures made? Will the protein travel through any organelles during its manufacture? Which ones? What would be the overall result if some part of the manufacture process went wrong, such that the protein ended up as nonfunctional?arrow_forwardTrace the path of a protein synthesized on a ribosome attached to the rough endoplasmic reticulum (rough ER) as the protein travels to the exterior of the cell. Name all the subcellular structures that the protein passes through. In answering this question, assume that the protein is already produced/synthesized (i.e. don't describe translation).arrow_forwardExplain the steps involved in the synthesis of a protein on membrane bound ribosomesarrow_forward
- Match the following: Forms part of the subunits for the protein synthesizing organelle A molecule that binds to a specific codon and specific amino acid simultaneously Attaches the correct amino acid to its transfer RNA Provides the energy needed for synthesis reactions Produced in the nucleus, this molecule specifies the exact sequence of amino acids of the protein to be made May be attached to the ER or scattered in the cytoplasm [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] SEXINVarrow_forwardWhat is the most likely pathway taken by a newly synthesized protein that will be secreted by a cell? C ER → Golgi → nucleus ER → lysosomes → vesicles that fuse with plasma membrane Golgi – ER → lysosome ER → Golgi - vesicles that fuse with plasma membranearrow_forwardBriefly describe the experiment carried out to demonstrate that proteins are capable of lateral movement in cell membranes.arrow_forward
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