Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Question
Chapter 5.9, Problem 3MQ
Summary Introduction
The plate count method is also called as viable cell count. It depends on the bacterial growth as colony on a solid medium. Once, the bacterial colonies become visible, the number of bacterial colonies can be calculated. Plating method is used in research laboratories to calculate the number of bacterial colonies formed in the Petri dishes, which enclosing the growth media.
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8. Transcription and Translation Practice: (Video 10-1 and 10-2)
A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense
DNA strand and label the 5' and 3' ends.
B. Use the antisense strand that you create in part A as a template to create the mRNA
transcript of the gene and label the 5' and 3' ends.
C. Translate the mRNA you produced in part B into the polypeptide sequence making sure
to follow all the rules of translation.
5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand)
4
What is the structure and function of Eukaryotic cells, including their organelles? How are Eukaryotic cells different than Prokaryotic cells, in terms of evolution which form of the cell might have came first? How do Eukaryotic cells become malignant (cancerous)?
What are the roles of DNA and proteins inside of the cell? What are the building blocks or molecular components of the DNA and proteins? How are proteins produced within the cell? What connection is there between DNA, proteins, and the cell cycle? What is the relationship between DNA, proteins, and Cancer?
Chapter 5 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 5.1 - Summarize the steps that lead up to binary fission...Ch. 5.1 - Define the term generation. What is meant by the...Ch. 5.2 - Prob. 1MQCh. 5.2 - Prob. 2MQCh. 5.2 - Prob. 3MQCh. 5.3 - Prob. 1MQCh. 5.3 - Prob. 2MQCh. 5.3 - Prob. 3MQCh. 5.4 - What are autolysins and why are they necessary?
Ch. 5.4 - What is the function of bactoprenol?
Ch. 5.4 - What is transpeptidation and why is it important?
Ch. 5.5 - What is a semilogarithmic plot and what...Ch. 5.5 - Prob. 2MQCh. 5.5 - Prob. 3MQCh. 5.6 - In which phase of the growth curve do cells divide...Ch. 5.6 - Prob. 2MQCh. 5.6 - Prob. 3MQCh. 5.7 - How do microorganisms in a chemostat differ from...Ch. 5.7 - What happens in a chemostat if the dilution rate...Ch. 5.7 - Do pure cultures have to be used in a chemostat?Ch. 5.8 - What are some of the problems that can arise when...Ch. 5.8 - Using microscopic techniques, how could you tell...Ch. 5.9 - Why is a viable count more sensitive than a...Ch. 5.9 - Describe how you would dilute a bacterial culture...Ch. 5.9 - Prob. 3MQCh. 5.10 - List two advantages of using turbidity as a...Ch. 5.10 - Describe how you could use a turbidity measurement...Ch. 5.11 - How does a hyperthermophile differ from a...Ch. 5.11 - Prob. 2MQCh. 5.11 - E. coli can grow at a higher temperature in a...Ch. 5.12 - Prob. 1MQCh. 5.12 - What molecular adaptations to cold temperatures...Ch. 5.13 - Which phylogenetic domain includes species with...Ch. 5.13 - How does the membrane structure of...Ch. 5.13 - What is Taq polymerase and why is it important?Ch. 5.14 - How does the concentration of H+ change when a...Ch. 5.14 - What terms are used to describe organisms whose...Ch. 5.15 - What is the aw of pure water? What is the lower...Ch. 5.15 - What are compatible solutes, and when and why are...Ch. 5.16 - How does an obligate aerobe differ from a...Ch. 5.16 - How does a reducing agent work? Give an example of...Ch. 5.16 - How does Superoxide dismutase or superoxide...Ch. 5.17 - Why is heat an effective sterilizing agent?Ch. 5.17 - What steps are necessary to ensure the sterility...Ch. 5.17 - Distinguish between the sterilization of...Ch. 5.18 - Define D10 and explain why the killing dose for...Ch. 5.18 - Prob. 2MQCh. 5.18 - Prob. 3MQCh. 5.19 - Distinguish between the antimicrobial effects of...Ch. 5.19 - Describe how the minimum inhibitory concentration...Ch. 5.19 - Distinguish between a sterilant, a disinfectant,...Ch. 5 - Prob. 1RQCh. 5 - Describe the role of proteins present at the...Ch. 5 - Prob. 3RQCh. 5 - Describe how new peptidoglycan subunits are...Ch. 5 - Prob. 5RQCh. 5 - Describe the growth cycle of a population of...Ch. 5 - How does a chemostat regulate growth rate and cell...Ch. 5 - How does a viable count differ from a total count?Ch. 5 - How can turbidity be used as a measure of cell...Ch. 5 - Prob. 10RQCh. 5 - Prob. 11RQCh. 5 - Concerning the pH of the environment and of the...Ch. 5 - How does a halophile maintain positive water...Ch. 5 - Prob. 14RQCh. 5 - Prob. 15RQCh. 5 - Contrast the terms thermal death time and decimal...Ch. 5 - Prob. 17RQCh. 5 - Prob. 18RQCh. 5 - Prob. 19RQCh. 5 - Describe the procedure for obtaining the minimum...Ch. 5 - Prob. 21RQCh. 5 - A medium was inoculated with 5 106 cells/ml of...Ch. 5 - Escherichia coli but not Pyrolobus fumarii will...Ch. 5 - In which direction (into or out of the cell) will...
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