Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Textbook Question
Chapter 55, Problem 9TYU
Level 3: Synthesis/Evaluation
9. DRAW IT (a) Draw a simplified global water cycle showing ocean, land, atmosphere, and runoff from the land to the ocean. Label your drawing with these annual water fluxes:
■ ocean evaporation, 425 km3
■ ocean evaporation that returns to the ocean as precipitation, 385 kin3
■ ocean evaporation that falls as precipitation on land, 40 km3
■ evapotranspiration from plants and soil that falls as precipitation on land, 70km3
■ runoff to the oceans, 40 km3
(b) What is the ratio of ocean evaporation that falls as precipitation on land compared with runoff from land to the oceans? (c) How would this ratio change during an ice age. and why?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 55 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Ch. 55.1 - Why is the transfer of energy in an ecosystem...Ch. 55.1 - WHAT IF? You are studying nitrogen cycling on the...Ch. 55.1 - MAKE CONNECTIONS Use the second law of...Ch. 55.2 - Why is only a small portion of the solar energy...Ch. 55.2 - How can ecologists experimentally determine the...Ch. 55.2 - Prob. 3CCCh. 55.2 - MAKE CONNECTIONS Explain how nitrogen and...Ch. 55.3 - If an insect that eats plant seeds containing 100...Ch. 55.3 - Prob. 2CCCh. 55.3 - WHAT IF? Detritivores are consumers that obtain...
Ch. 55.4 - DRAW IT For each of the four biogeochemical...Ch. 55.4 - Why does deforestation of a watershed increase the...Ch. 55.4 - WHAT IF? Why is nutrient availability in a...Ch. 55.5 - Prob. 1CCCh. 55.5 - WHAT IF? In what way is the Kissimmee River...Ch. 55 - Considering the second law of thermodynamics,...Ch. 55 - Prob. 55.2CRCh. 55 - Why would runners hove a lower production...Ch. 55 - If decomposers usually grow faster and decompose...Ch. 55 - In preparing a site for surface mining and later...Ch. 55 - Which of the following organisms is incorrectly...Ch. 55 - Prob. 2TYUCh. 55 - The discipline that applies ecological principles...Ch. 55 - Level 2: Application/Analysis 4. Nitrifying...Ch. 55 - Which of the following has the greatest effect on...Ch. 55 - Prob. 6TYUCh. 55 - Which of the following would be considered an...Ch. 55 - Prob. 8TYUCh. 55 - Level 3: Synthesis/Evaluation 9. DRAW IT (a) Draw...Ch. 55 - Prob. 10TYUCh. 55 - Prob. 11TYUCh. 55 - WRITE ABOUT A THEME: ENERGY AND MATTER...Ch. 55 - Prob. 13TYU
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