Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Chapter 55.2, Problem 4CC
MAKE CONNECTIONS Ø Explain how nitrogen and phosphorus, the nutrients that most often limit primary production, are necessary for the Calvin cycle to function in photosynthesis (see Concept 10.3).
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 55 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Ch. 55.1 - Why is the transfer of energy in an ecosystem...Ch. 55.1 - WHAT IF? You are studying nitrogen cycling on the...Ch. 55.1 - MAKE CONNECTIONS Use the second law of...Ch. 55.2 - Why is only a small portion of the solar energy...Ch. 55.2 - How can ecologists experimentally determine the...Ch. 55.2 - Prob. 3CCCh. 55.2 - MAKE CONNECTIONS Explain how nitrogen and...Ch. 55.3 - If an insect that eats plant seeds containing 100...Ch. 55.3 - Prob. 2CCCh. 55.3 - WHAT IF? Detritivores are consumers that obtain...
Ch. 55.4 - DRAW IT For each of the four biogeochemical...Ch. 55.4 - Why does deforestation of a watershed increase the...Ch. 55.4 - WHAT IF? Why is nutrient availability in a...Ch. 55.5 - Prob. 1CCCh. 55.5 - WHAT IF? In what way is the Kissimmee River...Ch. 55 - Considering the second law of thermodynamics,...Ch. 55 - Prob. 55.2CRCh. 55 - Why would runners hove a lower production...Ch. 55 - If decomposers usually grow faster and decompose...Ch. 55 - In preparing a site for surface mining and later...Ch. 55 - Which of the following organisms is incorrectly...Ch. 55 - Prob. 2TYUCh. 55 - The discipline that applies ecological principles...Ch. 55 - Level 2: Application/Analysis 4. Nitrifying...Ch. 55 - Which of the following has the greatest effect on...Ch. 55 - Prob. 6TYUCh. 55 - Which of the following would be considered an...Ch. 55 - Prob. 8TYUCh. 55 - Level 3: Synthesis/Evaluation 9. DRAW IT (a) Draw...Ch. 55 - Prob. 10TYUCh. 55 - Prob. 11TYUCh. 55 - WRITE ABOUT A THEME: ENERGY AND MATTER...Ch. 55 - Prob. 13TYU
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
- An unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forwardWhich of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forward
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