BROOKER BIOLOGY
5th Edition
ISBN: 9781307656152
Author: BROOKER
Publisher: MCG/CREATE
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Chapter 50.5, Problem 1CC
Summary Introduction
To explain: The individual as per the figure developed growth hormone disorder before or after puberty.
Introduction: Growth hormone is produced by the anterior lobe of pituitary gland. Growth hormone (GH) helps in stimulating the growth in vertebrates. An excessive amount of GH is secreted by the anterior pituitary gland in rare cases. This leads to pituitary gigantism, a kind of disorder characterized by increased height in an individual.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
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Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 50 Solutions
BROOKER BIOLOGY
Ch. 50.2 - Prob. 1CSCh. 50.3 - Prob. 1CSCh. 50.3 - Prob. 1EQCh. 50.3 - Prob. 2EQCh. 50.3 - Prob. 3EQCh. 50.4 - Prob. 1CCCh. 50.4 - Prob. 1CSCh. 50.4 - Prob. 2CCCh. 50.5 - Prob. 1CCCh. 50.5 - Prob. 2CC
Ch. 50 - Which is the defining feature of hormones? a. They...Ch. 50 - Steroid hormones are synthesized from ______blank...Ch. 50 - Prob. 3TYCh. 50 - Prob. 4TYCh. 50 - Prob. 5TYCh. 50 - Prob. 6TYCh. 50 - Which of the following pairs of hormones are...Ch. 50 - In invertebrates, molting of larvae is stimulated...Ch. 50 - Which of the following is true of the adrenal...Ch. 50 - Prob. 10TYCh. 50 - Prob. 1CQCh. 50 - Distinguish between type 1 and type 2 diabetes...Ch. 50 - Core Concept: Systems Organ systems often exert...Ch. 50 - Discuss the roles of hormones in growth and...Ch. 50 - Prob. 2COQ
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